| QIAO Qiao,ZHAO Xin-yu,LI Guang.Activation of NF-κB-HIF-1α pathway induced by X-ray irradiation in human lymphoma cells[J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):25-28 |
| Activation of NF-κB-HIF-1α pathway induced by X-ray irradiation in human lymphoma cells |
| Received:February 28, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.007 |
| KeyWords:Lymphoma NF-κB HIF-1α Ionizing radiation |
| FundProject:辽宁省教育厅基金(2009A754) |
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| Abstract:: |
| Objective To observe the effect of X-ray radiation on NF-κB-HIF-1α pathway activation in three malignant lymphoma and to explore the mechanism of radioresistance mediated by this pathway. Methods Expression levels of p-P65, IKK and HIF-1α in Namalwa, Ramos and Raji cells were determined by Western blot 1,4,10 and 20 h after 5 Gy X-ray irradiation. The effect of QNZ on HIF-1α expression was also observed. Results Expression level of IKK protein was up-regulated 4 h after irradiation in the 3 lymphoma cell strains(t=8.01, 5.14, 5.42, P<0.01), followed by up-regulation of p-P65 protein expression level at 10 h and HIF-1α at 10-20 h after X-ray irradiation (t=11.25, 17.43, 22.09, P<0.01). Pretreatment of QNZ 24 h before X-ray irradiation significantly reduced the up-regulation of HIF-1α protein expression induced by simple ion radiation (t=18.69, 19.35, 12.26,P<0.01). Conclusion NF-κB - HIF-1α pathway was activated in human lymphoma cells after ion radiation and may be involved in the mechanism of radioresistance. |
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