| DU Li,MA Qiong,CUI Yu-fang,et al.Changes and significance of CD4+CD25+ regulatory T cells in γ-ray irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):21-24 |
| Changes and significance of CD4+CD25+ regulatory T cells in γ-ray irradiated mice |
| Received:April 26, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.006 |
| KeyWords:Ionizing radiation Peripheral blood Spleen Regulatory T cells |
| FundProject:国家自然科学基金(30900381);军队医药卫生"十一五"基金(09MA026) |
| Author Name | Affiliation | | DU Li | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | MA Qiong | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | CUI Yu-fang | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | SUN Rong | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | PAN Xiu-jie | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | XIE Ling | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | DONG Bo | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | XIAO Feng-jun | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | GAO Zhuang-zhuang | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | MAO Jian-ping | Beijing Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract:: |
| Objective To observe the effect of γ-ray irradiation on CD4+CD25+ regulatory T cells (Tregs), and to investigate the mechanism of immune injury induced by irradiation. Methods The thymus and spleen of C57BL/6 mice were taken and weighted 1-28 d after γ-ray irradiation, and the organ coefficients were calculated. The amount of mouse peripheral WBC measured, CD4+T cells and Tregs in peripheral and splenic were analyzed by flow cytometry. Results Coefficients of mouse thymus and spleen decreased significantly 1 d post irradiation, and reached to the bottom at 7 d. Coefficients did not recover to control level 28 d after radiation. Peripheral WBC continuously decreased and reached the bottom at 7 d, and did not recover to control level up to 28 d postirradiation. Peripheral CD4+ T lymphocyte temporally reduced at 1 d, while it increased at 7 d, and it approached to control level at 28 d after radiation. Splenic CD4+ T cells slightly reduced at 7 d however, they basically maintained as the same level as control 14 d and 28 d after radiation. Peripheral Tregs ascended at 1 d and reached the peak at 7 d, and reduced at 14 d and 28 d postirradiation, although they still were significantly higher than those of control group. At the same time, splenic Tregs increased significantly and achieved peak value at 1 d, and then gradually decreased and reached the minimum at 28 d after irradiation, which were significantly lower than those of control group(t=2.731, P<0.05). Conclusions Mouse thymus and spleen were injured severely, and the number of immunocytes decreased after 6 Gy whole body γ-ray irradiation. However, Tregs with immunosuppressive action increased significantly postirradiation, revealing that Tregs were closely correlated with immune function depression and immunomodulation imbalance induced by ionizing radiation. |
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