| SUN Xiu-jin,CUI Feng-mei,HUANG Cheng-cheng,et al.Differential expression profiles of microRNAs in liver of 60Co γ-ray irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):13-16 |
| Differential expression profiles of microRNAs in liver of 60Co γ-ray irradiated mice |
| Received:July 13, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.004 |
| KeyWords:γ-rays Irradiation miRNA Microarray Bioinformatic analysis |
| FundProject:国家自然科学基金(30800922);江苏省高校自然科学研究项目(08KJD310007);2009年教育部"长江学者和创新团队发展计划资助"项目(IRT0849) |
| Author Name | Affiliation | | SUN Xiu-jin | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | CUI Feng-mei | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | HUANG Cheng-cheng | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | HU Ming-jiang | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | WANG Dao-jin | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | TU Yu | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China |
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| Abstract:: |
| Objective To investigate the differential expression profiles of microRNAs in the liver of 60Co γ-ray irradiated mice using microRNA microarray and to explore their main functions by bioinformatic analysis. Methods After SPF C57BL/6J mice expose to 4 Gy-single whole body radiation,total number of peripheral WBC and the fMNPCE were measured at 3 d. The differentially expressed miRNAs in mouse liver were detected with miRNA microarray. miRNA-124 and miR-34a were confirmed by real time RT-PCR assay. Bioinformatic analysis was applied to explore target genes and the main functions of the differential expressed miRNAs. Results Compared with control group, the total number of peripheral WBC decreased(t=2.87, P<0.05), while the fMNPCE in bone marrow increased(t=-2.91, P<0.05) after 4 Gy γ-ray irradiation. miRNA microarray revealed that 17 miRNAs were differentially expressed, in which 9 up-regulated, 8 down-regulated. The expression levels of miR-124 and miR-34a were coincident with the result of real time RT-PCR.GO analysis showed that some pathways including adherens junction and cell cycle were suppressed, while some immune-related pathways were activated. Conclusions miR-34a and miR-194 were involved in the regulation of acute radiation damage,some other miRNAs including miR-124、miR-382 and miR-92a* also played important roles in radiation process. |
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