| CHEN Shu-bo,TIAN Ye,LIU Chun-feng,et al.Gene expression profile of oligodendrocytes in vitro in early stage after ionizing irradiation[J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):10-12 |
| Gene expression profile of oligodendrocytes in vitro in early stage after ionizing irradiation |
| Received:December 07, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.003 |
| KeyWords:Oligodendrocyte Radiation Gene expression Gene chips |
| FundProject:国家自然科学基金(30170287, 30870740);江苏省"六大人才高峰"资助项目 |
| Author Name | Affiliation | | CHEN Shu-bo | Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | TIAN Ye | Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | LIU Chun-feng | Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | LI Chun-peng | Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | ZHANG Ye | Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | XIA Chun-lin | Department of Radiotherapy & Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China |
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| Abstract:: |
| Objective To characterize the gene expression in acute phase of irradiated oligodendrocytes (OL) in vitro. Methods The total RNA was extracted from irradiated OLs with 10 Gy by 6 MV X-rays at 1 and 4 h. The Affymetrix RAT 230 2.0 microarray were used to evaluate and screen the gene expression profile. The quantitative real-time RT-PCR was performed to validate the microarray results of selected myelin basic protein (MBP) and neural cell adhesion molecule 1 (NCAM-1) genes. Results Compared with un-irradiated OLs, there were 1079 different expressed genes in irradiated cells. Those genes were classified in 79 categories based on the functional classification. Some familiar genes associated with OL cellular physiological process, apoptosis, cell cycle control, metabolism, cell communication and receptor binding were included. Compared with the microarray results, the coincidence rate of real-time RT-PCR was 91.7%. The down-regulation of MBP and up-regulation of NCAM 1 gene expression were confirmed. Conclusions Radiation-induced changes in gene expression in OLs took place in acute phase and influenced by time-course. The changes of MBP and NCAM 1 gene expression may play a key role in the pathogenesis of radiation-induced demyelination. |
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