| SUN Wen-jie,YU Hai-jun,XIONG Jie,et al.Anti-tumor effect of adenovirus-mediated suicide gene therapy under control of tumor-specific and radio-inducible chimeric promoter in combination with γ-ray irradiation in vivo [J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):6-9 |
| Anti-tumor effect of adenovirus-mediated suicide gene therapy under control of tumor-specific and radio-inducible chimeric promoter in combination with γ-ray irradiation in vivo |
| Received:June 28, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.002 |
| KeyWords:Gene-radiotherapy Hepatocellular carcinoma Adenovirus Human telomerase reverse transcriptase Chimeric promoter |
| FundProject:国家自然科学基金(30672438); 湖北省自然科学基金创新项目(2006ABC009);武汉市攻关项目(200960323129) |
| Author Name | Affiliation | | SUN Wen-jie | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | YU Hai-jun | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | XIONG Jie | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | XU Yu | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | LIAO Zheng-kai | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | ZHOU Fu-xiang | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | XIE Cong-hua | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China | | ZHOU Yun-feng | Department of Radiation and Medical Oncology, Zhongnan Hospital, Hubei Key Laboratory of Tumor Biological Behaviors, Wuhan University, Wuhan 430071, China |
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| Abstract:: |
| Objective To detect the selective inhibitory effects of irradiation plus adenovirus-mediated horseradish peroxidase(HRP)/indole-3-acetic acid(IAA) suicide gene system using tumor-specific and radio-inducible chimeric promoter on human hepatocellular carcinoma subcutaneously xenografted in nude mouse. Methods Recombinant replicated-deficient adenovirus vector containing HRP gene and chimeric human telomerase reverse transcriptase(hTERT) promoter carrying 6 radio-inducible CArG elements was constructed. A human subcutaneous transplanting hepatocellular carcinoma (MHCC97 cell line) model was treated with γ-ray irradiation plus intra-tumor injections of adenoviral vector and intra-peritoneal injections of prodrug IAA. The change of tumor volume and tumor growth inhibiting rate, the survival time of nude mice, as well as histopathology of xenograft tumor and normal tissues were evaluated. Results Thirty one days after the treatment, the relative tumor volumes in the negative, adenovirus therapy, irradiation, and combination groups were 49.23±4.55, 27.71±7.74, 28.53±10.48 and 11.58±3.23,respectively. There was a significantly statistical difference among them(F=16.288,P<0.01). The inhibition effect in the combination group was strongest as compared with that in other groups, and its inhibition ratio was 76.5%. The survival period extended to 43 d in the combination group, which showed a significantly difference with that in the control group(χ2=18.307,P<0.01). The area of tumors necrosis in the combination group was larger than that in the other groups, and the normal tissues showed no treatment-related toxic effect in all groups. However, multiple hepatocellular carcinoma metastases were observed in the liver in the control group, there were a few metastases in the monotherapy groups and no metastasis in the combination group. Conclusions Adenovirus-mediated suicide gene therapy plus radiotherapy dramatically could inhibit tumor growth and prolong median survival time. It might provide a promising therapeutic modality for hepatocellular carcinoma therapy. |
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