| GOU Qiao,ZHANG Wei,WANG Chun-yan,et al.Antioxidant ability and radiosensitivity in malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle irradiation[J].Chinese Journal of Radiological Medicine and Protection,2011,31(1):1-5 |
| Antioxidant ability and radiosensitivity in malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle irradiation |
| Received:June 21, 2010 |
| DOI：10.3760/cma.j.issn.0254-5098.2011.01.001 |
| KeyWords:Human bronchial epithelial cell line α-particle Antioxidant ability Radio-sensitivity Carcinogenesis |
| FundProject:国家自然科学基金(81000862);中国科学技术部社会公益研究专项(2005DIB1J087);卫生行业科研专项(200802018) |
| Author Name | Affiliation | | GOU Qiao | National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention, Beijing 100088, China | | ZHANG Wei | National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention, Beijing 100088, China | | WANG Chun-yan | National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention, Beijing 100088, China | | SU Xu | National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention, Beijing 100088, China |
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| Abstract:: |
| Objective To investigate the antioxidant ability and radiosensitivity in the malignant transformed human bronchial epithelial cell line BEP2D induced by α-particle exposure. Methods Glutathione Peroxidase (GPX), Catalase (CAT) and Glutathione (GSH) assay kits were employed to detect GPX and CAT enzyme abilities and the levels of GSH in BEP2D, RH21 (passage 21 of α-particle-irradiated BEP2D cells), and BERP35T-1 cells (derived from nude mice bearing malignant transformed cells generated from cells of passage 35 of α-particle-irradiated BEP2D cells). MTT assay were used to test the growth rate of BEP2D, RH21 and BERP35T-1 cells treated with 0, 30, 60, 90, 120, and 150 μmol/L H2O2. Colony-forming test and MTT assay were used to examine the cell survival fraction and the growth rate of BEP2D, RH21 and BERP35T-1 cells exposed to 0, 2, 4, and 8 Gy of γ-rays, respectively. Results GPX and CAT enzyme activities in RH21 and BERP35T-1 cells were obviously lower than in BEP2D(t=5.75-67.92,P<0.05). CAT enzyme activity in BERP35T-1 was lower than that in RH21 cells(t=22.25,P<0.01). Compared to BEP2D cells, decreased level of GSH was detected in BERP35T-1 cells(t=7.76,P<0.05), but there was no change in RH21. After treatment with 30, 60, 90, 120, and 150 μmol/L H2O2, the growth rates of BEP2D were all higher than those of BERP35T-1 cells(t=10.37-58.36,P<0.01). Meanwhile, the growth rates of BEP2D were all higher than those of RH21 cells after treatment with 60, 90, 120, and 150 μmol/L H2O2(t= 29.90-84.68,P<0.01). In addition, compared to BEP2D cells, decreased cell survival fraction and growth rate of BERP35T-1 cells were observed after irradiation with 2, 4, and 8 Gy of γ-rays(t=5.87-34.17,P<0.05). The cell survival fraction and growth rate of RH21 were all lower than those of BEP2D cells at 4 and 8 Gy post-irradition(t= 6.33- 45.00,P<0.05). Conclusion The function of antioxidant system decreased in the α-particle-induced transformed cells, which could contribute to the acceleration of cellular malignant transforming process and radiosensitivity. |
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