| ZHOU Chong,ZHOU Ju-ying,WANG Li-li,YU Zhi-ying,XU Xiao-ting,QIN Song-bing,NIE Bin.Study of antisense oligonucleotide miR-21 on radiosensitivity of SHG-44 in vitro[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):701-704 |
| Study of antisense oligonucleotide miR-21 on radiosensitivity of SHG-44 in vitro |
| Received:December 18, 2009 |
| DOI： |
| KeyWords:Glioma Radiosensitivity miR-21 Cell Cycle Apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | ZHOU Chong | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | | | ZHOU Ju-ying | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | zhjuying@sohu.com | | WANG Li-li | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | | | YU Zhi-ying | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | | | XU Xiao-ting | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | | | QIN Song-bing | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | | | NIE Bin | Department of Radiation Oncology, First Hospital Affiliated to Soochow University, Suzhou 215006, China | |
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| Abstract:: |
| Objective To investigate the radiosensitizing effect of knock-down the expression of miR-21 on human SHG-44 glioma cells and explore the possible mechanism. Methods Antisense oligonuleotides of miR-21,mediated by LipofectamineTM 2000,were transfected to SHG-44 cells.Three groups were:blank control group(mock group),negative control and antisense transfected group(AS-miR-21 gorup).Cells of each group were irradiated with 6 MeV X-rays at the doses of 0,1,2,4,6 and 8 Gy.Dose-suvivial curve was established by colony-forming assay.The influence of AS-miR-21 on cell cycle and cell apoptosis was analyzed by flow cytometry assay after 6 Gy irradiation.Results The value of D0 and Dq of AS-miR-21 group declined obviously compared with the mock group and negative control group.Flow cytometric analysis showed that cell cycle distribution changed(G0/G1 phase arrest,S phase decreased) after transfected with AS-miR-21(t =8.18,-4.52, P <0.05).The sensitization enhancement ratios of D0 and Dq were 1.32 and 2.10 respectively.Apoptosis assay showed the early apoptosis rate was significantely increased in AS-miR-21、irradition alone and combined group than mock control group(t =20.14,11.11,50.07, P <0.05).Conclusions AS-miR-21 can enhance the radiosensitivity of human glioma cells SHG-44 by promoting cell apoptosis and faciliating cell cycle redistribution. |
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