| PIAO Chun-nan,TIAN Mei,LIU Jian-xiang,RUAN Jian-lei,SU Xu.Effects of radon and its progeny on the expression and mutation of p53 in lung tissues of mice[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):696-700 |
| Effects of radon and its progeny on the expression and mutation of p53 in lung tissues of mice |
| Received:January 07, 2010 |
| DOI： |
| KeyWords:Radon and its progeny Lung injury Apoptosis p53 Mutation |
| FundProject:科技部科研院所社会公益研究专项(2005DIB1J087) |
| Author Name | Affiliation | E-mail | | PIAO Chun-nan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | TIAN Mei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To explore the effect of radon and its progeny on the expression and mutations of p53 in lung tissue of mouse model.Methods Apoptosis was detected by terminal deoxynucleotidy transferase-mediated dUTP-biotin nick end labeling. The expression of p53 gene was analyzed by immunohistochemistry, Western blot and realtime-PCR. PCR-SSCP was used to detect the mutation of p53 in lung tissues.Results Compared with those in the control group, the apoptotic index were increased significantly in 30 WLM and 60 WLM groups(t=18.11,-10.30,P<0.05). The p53 protein was increased significantly(t=-11.08,P<0.05;t=-7.00,P<0.05))in 30 WLM and 60 WLM groups. The mutation of p53 gene was not detected in lungs of radon-exposure mice. Conclusions Lung and bronchus might be the targets of radon and its progeny, and p53 gene plays an important role in the progression of radon-induced lung injury. |
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