二氢青蒿素对人宫颈癌细胞株照射后周期的影响及相关机制

陈遐林; 纪蓉; 曹建平; 朱巍; 樊赛军; 王建芳

CHEN Xia-lin,JI Rong,CAO Jian-ping,et al.Effect of dihydroartemisinin on the cell cycle progress of irradiated human cervical cancer cell line and its mechanism[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):692-695

Effect of dihydroartemisinin on the cell cycle progress of irradiated human cervical cancer cell line and its mechanism

Received:December 14, 2009

DOI：

KeyWords:p53 Cell cycle Human HeLa cells Human SiHa cells Dihydroartemisinin

FundProject:国家自然科学基金(30670632); 教育部长江学者和创新团队发展计划项目(IRT0849)

Author Name	Affiliation	E-mail
CHEN Xia-lin	Radiotherapy center of the People's Hospital of Shaoxing, Shaoxing 312000, China
JI Rong	Radiotherapy center of the People's Hospital of Shaoxing, Shaoxing 312000, China
CAO Jian-ping	苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室
朱巍	苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室
樊赛军	苏州大学医学部放射医学与公共卫生学院江苏省放射医学与防护重点实验室	jpcao@suda.edu.cn
王建芳	Radiotherapy center of the People's Hospital of Shaoxing, Shaoxing 312000, China

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Abstract::

Objective To observe the changes of cell cycle on cancer cells after dihydroartemisinin and X-ray irradiation. Methods Human HeLa cells of cervical cancer with p53 mutation was used and human SiHa cells of cervical cancer with wild p53 was used as control. Flow cytometry was used to detect the effect of dihydroartemisinin (20 and 100 μmol/L) and irradiation (6 Gy) on cell cycle. Western blot was used to measure the levels of cell cycle protein. Results G₂ arrest was observed in irradiated HeLa cells, which the proportion of cells in G₂ phase was increased from 14.45% to 73.58% after 6 Gy X-ray irradiation, but it was abrogated by dihydroartemisinin from 73.58% to 48.31% in HeLa cells, and it had no change on the SiHa cells. The elevated Wee1 protein and the lowered Cyclin B1 protein were observed with the G₂ arrest severity. The expression of radiation-induced Wee1 protein was suppressed and the Cyclin B1 protein was increased after dihydroartemisinin treatment, which was in accordance with the abrogation of radiation-induced G₂ delay. Conclusions The main effect of irradiation on cell cycle of p53 mutated HeLa cells is G₂ arrest. Dihydroartemisinin could abrogate it, which is associated with the changes of Wee1 protein and Cyclin B1 protein. In Siha cells, the main effect of irradiation on cell cycle is G₁ arrest, and dihydroartemisinin has no effect on it.

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