| LUO Ming,WU Gang,FAN Li,et al.Inhibitory effect of Corilagin on the inflammatory response of irradiated microglia BV-2 cells[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):682-686 |
| Inhibitory effect of Corilagin on the inflammatory response of irradiated microglia BV-2 cells |
| Received:February 09, 2010 |
| DOI： |
| KeyWords:Corilagin Microglia BV-2 NF-κB Radiation encephlopathy |
| FundProject:国家自然科学基金(30800283) |
| Author Name | Affiliation | E-mail | | LUO Ming | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430023, China | | | WU Gang | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430023, China | | | FAN Li | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430023, China | | | ZHANG Rui-guang | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430023, China | | | REN Jing-hua | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430023, China | | | DONG Ji-hua | 华中科技大学同济医学院附属协和医院中心实验室 | | | DONG Xiao-rong | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430023, China | xr.dong@gmail.com |
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| Abstract:: |
| Objective To explore the inhibitory effects of Corilagin on the production of pro-inflammatory cytokines in microglia induced by radiation.Methods The cytotoxicity of Corilagin was measured by MTT assay. Microglia BV-2 cells were irradiated 0 or 32 Gy after pretreated with Corilagin for 12 hours. Realtime-PCR was used to detect the mRNA levels of inflammatory cytokines, such as IL-1β, TNF-α on several time-points. The content of nitric oxide (NO) was determined with nitrate reductase method. The translocation of NF-κB was measured by Western blot and immunocytochemical stain. Confocal microscopy was used to observe the expression of Iba-1 and Nemo. Results No cytotoxicity was detected on BV-2 cells with 1-10 μg/ml Corilagin. Iba-1 expression in microglia cells was activated by irradiation, the expression levels of inflammatory cytokines, such as IL-1β, TNF-α and NO were also elevated. Whereas, the production of IL-1β, TNF-α in activated microglia cells was significantly inhibited with 5 μg/mL corilagin (tIL-1β=6.341, tTNF-α=3.411, tNO=3.134, P <0.05). Corilagin significantly inhibited the expression of Nemo and the translocation of NF-κB p65. Conclusion Corilagin could inhibit the activation of irradiated microglia cells and down-regulate the expression of inflammatory cytokines, via inhibition of the NF-κB signaling pathway. |
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