| LI Yu-wen,FENG Jiang-bin,LU Xue,et al.Expression changes of in human mitochondrial COX genes in human lymphocytes after exposed by 60Co γ-rays[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):677-681 |
| Expression changes of in human mitochondrial COX genes in human lymphocytes after exposed by 60Co γ-rays |
| Received:December 17, 2009 |
| DOI： |
| KeyWords:60Co γ-rays Human lymphoblastoid cell line Mitochondrion COX Gene expression |
| FundProject:国家自然科学基金(30870749, 30570551) |
| Author Name | Affiliation | E-mail | | LI Yu-wen | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | FENG Jiang-bin | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | LU Xue | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | FAN Li | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | CHEN De-qing | National Institute for Radiological Protection, China CDC, Beijing 100088, China | | | LIU Qing-jie | National Institute for Radiological Protection, China CDC, Beijing 100088, China | qjliu@nirp.cn |
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| Abstract:: |
| Objective To explore the changes of human mitochondrial COXⅠ, COXⅡ and COXⅢ genes expression induced by ionizing irradiation.Methods Changes of human COX genes expression were detected by RT-PCR and Real-time PCR 8 h after the irradiation in human lymphoblastoid cell lines, which were exposed to 1-10 Gy60Co γ-rays. The protein levels were detected by flow cytometry and the COX activity was measured by colorimetry. The dose-effect relationships between the expression changes of the genes and the doses were established. The changes of these genes expression were also analyzed at different post-radiation time-points between 0.5 h and 72 h after irradiation of 5 Gy in order to explore the time-effect.Results The expression of 3 genes at mRNA level was up-regulated. A good dose-effect relationship was showed for COXⅠand COXⅢ at dose range of 0-3 Gy and 0-8 Gy for COXⅡ (F COXⅠ=116.62, FCOXⅡ=17.89, FCOXⅢ=8.20, P<0.05). For the time-effect after irradiation, the gene expression levels of COXⅡ and COXⅢ genes were up-regulated and the peak change occurred at 4 h after irradiation. For COXⅠgene, the mRNA expression levels were down-regulated during 0.5-72 h (FCOXⅠ=31.99, FCOXⅡ=19.47, FCOXⅢ=20.64, P<0.05). At the protein level, the levels of COXⅠ and COXⅡ were lowered in lower doses and enhanced in higher doses, and the levels of COX Ⅲ were decreased at all dose levels (FCOXⅠ=16.96, FCOXⅡ=32.5, FCOXⅢ=6.51, P<0.05). The protein levels of COXⅠ and COXⅡ were enhanced during 4-72 h and 8-72 h respectively after 5 Gy irradiation (FCOXⅠ=14.68, FCOXⅡ=17.18, FCOXⅢ=2.52, P<0.05). The activities of COX were lowered at different dose levels and different time-points.Conclusions Ionizing radiation might induce the changes in mitochondrial COXⅠ, COXⅡ and COXⅢ gene expression, and lead to the reduction of the COX activities. |
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