| WAN Jian-mei,ZHANG You-jiu,FAN Wo,ZHU Ran,NING Ping,CUI Feng-mei.Comparison of 3H-TdR and 125I-UdR incorporation on the proliferation effect of lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):658-660 |
| Comparison of 3H-TdR and 125I-UdR incorporation on the proliferation effect of lymphocytes |
| Received:July 13, 2010 |
| DOI： |
| KeyWords:Lymphocytes Lymphoma cells 3H-TdR 125I-UdR Incorporating fraction |
| FundProject:江苏省卫生厅医学科技发展基金(H200341);苏州市社会发展科技计划资助(SS0416) |
| Author Name | Affiliation | E-mail | | WAN Jian-mei | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | ZHANG You-jiu | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | yjzhang@suda.edu.cn | | FAN Wo | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | ZHU Ran | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | NING Ping | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | | | CUI Feng-mei | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China | |
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| Abstract:: |
| Objective To compare the incorporation method of 3H-TdR and 125I-UdR on determining the proliferation effect of lymphocytes.Methods The proliferation effects of lymphocyte and Daudi lymphoma cells were estimated by 3H-TdR and 125I-UdR incorporation. Results The incorporating fraction of 3H-TdR and125I-UdR into lymphocyte was 20.95%±1.06% and 1.00%±0.04%, respectively, and the incorporating fraction for the lymphoma cells was 29.94%±4.10% and 6.02%±0.73%,respectively. The incorporation fractions of 3H-TdR into lymphocyte and lymphoma cells were much higher than those of 125I-UdR, but the incorporating fractions of 3H-TdR or 125I-UdR into the lymphoma cells were much higher than those of lymphocytes. Conclusions For lymphocytes, 125I-UdR cannot substitute 3H-TdR as a tracer agent. But for lymphoma cells, whether 125I-UdR could be replace 3H-TdR or not needs further research. |
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