| JIAO Yang,ZHU Wei,WANG Li-li,ZHANG Yu-song,XU Jia-ying,FAN Sai-jun.Preliminary study on high mobility group box 1 as a biological dosimeter of ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):647-649 |
| Preliminary study on high mobility group box 1 as a biological dosimeter of ionizing radiation |
| Received:July 13, 2010 |
| DOI： |
| KeyWords:HMGB1 Ionizing irradiation Biological dosimeter |
| FundProject:江苏省高校自然基金(SZ126821) |
| Author Name | Affiliation | E-mail | | JIAO Yang | Key Laboratory of Radiation Oncology, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Soochow University, Suzhou 125123, China | | | ZHU Wei | Key Laboratory of Radiation Oncology, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Soochow University, Suzhou 125123, China | | | WANG Li-li | Key Laboratory of Radiation Oncology, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Soochow University, Suzhou 125123, China | | | ZHANG Yu-song | Key Laboratory of Radiation Oncology, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Soochow University, Suzhou 125123, China | | | XU Jia-ying | Key Laboratory of Radiation Oncology, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Soochow University, Suzhou 125123, China | | | FAN Sai-jun | Key Laboratory of Radiation Oncology, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Public Health, Soochow University, Suzhou 125123, China | sjfan@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the probability of high mobility group box 1 protein as a biological dosimeter of ionizing radiation. Methods The medium of cultured human fibroblast cell line GM was collected 24 h after exposed to 60Co γ-rays, and HMGB1 protein concentration was detected by ELISA assay, and the dose-effect curve was then fitted. The levels of HMGB1 protein was also detected after exposed to 4 Gy irradiation at 24, 48 and 72 h. Results The level of HMGB1 protein in culture medium was increased in a dose-dependant way, and the dose-effect curve of HMGB1 level for 24 h after irradiation fitted the linear model y= 0.5655 +0.0358x (r=0.9339). After exposure to 4 Gy irradiation, the levels of HMGB1 protein in the culture medium were also increased time-dependently. Conclusion The release of HMGB1 protein to neoplasm is reactivated by irradiation in GM cell. It is necessary to further the studies on HMGB1 as biological dosimeter for radiation injury protection and therapy. |
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