| LIU Ping,TU Yu.Study on the protective effect of MgSO4 on the radiation-induced neural stem cell injury[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):639-642 |
| Study on the protective effect of MgSO4 on the radiation-induced neural stem cell injury |
| Received:July 13, 2010 |
| DOI： |
| KeyWords:Neural stem cell Ionizing radiation Magnesium sulfate |
| FundProject:国家自然科学基金(30670638);2009年教育部"长江学者和创新团队发展计划资助"项目(IRT0849) |
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| Abstract:: |
| Objective To explore the neuroprotective effect of magnesium sulfate on radiation-induced neural stem cell injury. Methods Brain tissue was obtained from new-born sprague-dawley rats within 24 hours, and the cerebral hemisphere was dissociated to culture the neural stem cells. After being identified by immunofluorescence method, the neural stem cells were randomly divided into 3 groups as blank control group, experimental control group and experimental group. The neural stem cells of experimental control group and experimental group were irradiated with 2 or 4 Gy of gamma rays. The proliferation and the cell cycle of neural stem cells were detected at different time-points ranging from 24 h, 48 h, 72 h after irradiation with CCK-8 and FCM. Results Compared with the blank control group, the proliferation rate of experimental control group was significantly reduced (t=5.33-8.44,P<0.05), and the G1 phase arrest of experimental control group was significantly enhanced (t=30.60-71.22,P<0.05). Compared with the experimental control group, the proliferation of experimental group significantly increased excluding that of 24 h (t=2.45-4.71,P<0.05),the apoptosis rate of experimental group significantly decreasd (t=6.73-41.12,P<0.05), which was closer to the blank control group. Conclusion Magnesium sulfate can alleviate the injury of proliferation and decrease the cell apoptosis in the early stage after irradiation. |
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