| WANG Ming-ming,SI Yuan,TAO Xin-quan,ZHANG Wu-kui,XIE Qiang.Effect of 18F-2-deoxy-2-fluoro-d-glucose on proliferation of Lewis lung cancer cell[J].Chinese Journal of Radiological Medicine and Protection,2010,30(5):568-571 |
| Effect of 18F-2-deoxy-2-fluoro-d-glucose on proliferation of Lewis lung cancer cell |
| Received:January 14, 2010 |
| DOI： |
| KeyWords:18F-FDG Apoptosis Bcl-2 protein Bax protein |
| FundProject:安徽省教育厅自然科学基金(2006KJ3123) |
| Author Name | Affiliation | E-mail | | WANG Ming-ming | Department of Nuclear Medicine,Anhui Medical University, Hefei 230032,China | wmgene@163.com | | SI Yuan | Department of Nuclear Medicine,Anhui Medical University, Hefei 230032,China | | | TAO Xin-quan | Department of Nuclear Medicine,Anhui Medical University, Hefei 230032,China | | | ZHANG Wu-kui | Department of Nuclear Medicine,Anhui Medical University, Hefei 230032,China | | | XIE Qiang | 安徽省立医院PET-CT中心 | | | 谢吉奎 | 安徽省立医院PET-CT中心 | |
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| Abstract:: |
| Objective To investigate the influence of 18F-FDG on the proliferation of Lewis lung cancer cell line,and to elucidate its possible mechanism. Methods Morphological changes of cells after culture for 24 h at different concentrations of 0, 0.37, 1.85, 3.70 and 7.4 (×106) Bq/ml of 18F-FDG were observed by using inverted microscopy and electron microscopy. The apoptosis and phase distribution of cell cycle of irradiated cells were analyzed with flow cytometry. DNA synthesis of irradiated cells was assayed by3H-TdR incorporation. Lipid peroxidation was measured by chromometry and expression of Bcl-2 and Bax proteinwas measured by immunohistochemical technique. Results Exposed to (0-7.40)×106 Bq/ml of 18F-FDG for 24 h, the cumulative absorbed doses delivered to cells in five groups were 0, 0.11, 0.55, 1.10 and 2.20 Gy, respectively. Irradiated cells showed morphological changes of apoptosis. The apoptosis rate of irradiated cells was increased from (4.05±0.01)% to(25.6±0.28)%(t=188, P<0.01).3H-TdR incorporation rate was decreased from 100% to(22.0±0.51)% ( t=27.6, P<0.05). The levels of MDA in cells were augmented from (0.08±0.03)to (0.67±0.12)μmol/L(t=11.7, P<0.01). Cell cycle arrest was found in G2/M phase with the increasing doses from 0 to 2.20 Gy. The expression of Bcl-2 protein was decreased while that of Bax protein increased. Conclusions 18F-FDG could induce the apoptosis of cells and inhibit the proliferation of cells. |
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