| ZHOU Ju-ying,XU Xiao-ting,LI Xiao-qing,et al.Radiosensitization of celecoxib on human lung adenocarcinoma cell line A549 and inhibition of migration ability in vitro[J].Chinese Journal of Radiological Medicine and Protection,2010,30(5):564-567 |
| Radiosensitization of celecoxib on human lung adenocarcinoma cell line A549 and inhibition of migration ability in vitro |
| Received:October 12, 2009 |
| DOI： |
| KeyWords:Lung neoplasms Radiosensitivity Cyclo-oxygenase-2 (COX-2) Metalloproteinase-2 (MMP-2) |
| FundProject:江苏高校省级重点实验室开放研究课题(KJS0723) |
| Author Name | Affiliation | E-mail | | ZHOU Ju-ying | Department of Radiation Oncology, First Affiliated Hospital of Soochow University,Suzhou 215006,China | | | XU Xiao-ting | Department of Radiation Oncology, First Affiliated Hospital of Soochow University,Suzhou 215006,China | | | LI Xiao-qing | 苏州市立医院东 区放疗科 | | | WANG Li-li | Department of Radiation Oncology, First Affiliated Hospital of Soochow University,Suzhou 215006,China | | | QIN Song-bing | Department of Radiation Oncology, First Affiliated Hospital of Soochow University,Suzhou 215006,China | | | TU Yu | 苏州大学放射医学与公共卫生学院 | tuyu@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the effects of radiosensitivity enhancement and inhibition of migration ability of human lung adenocarcinoma cells by celecoxib, a selective cyclooxygenase(COX)-2 inhibitor.Methods Human lung adenocarcinoma cells of the line A549 were cultured and then inoculated into six-well plates and randomly divided into 4 groups:control group, celecoxib group administered with celecoxib at the subtoxic doses 30 and 50 μmol/L, irradiated group exposed to 0, 1, 2, 4, 6, or 8 Gy by linear accelerator, and combined treatment (celecoxib+irradiation) group. The radiosensitizing effect of celecoxib was assessed by clonogenic cell survival test.The migration ability of the A549 cells was measured by scratch-wound test and the content of metalloproteinase-2 (MMP-2) in culture supernatant was detected with ELISA.Results The sensitization enhancement ratio of the celexib group was increased dose-dependently. The values of D0, Dq, SF-2 and D0.01 of the celecoxib+irradiation group were all significantly lower than those of the irradiated group. Scratch-wound test showed that the no-scratch area of the celecoxib+irradiation group and celecoxib group were all significantly wider than those of the mere irradiation and control groups and there was a dose-dependent manner, and the no-scratch area of the celecoxib+irradiation group was wlider than that of the celecoxib group. ELISA showed that the MMP-2 levels in the supernatant of the celecoxib group and celecoxib+irradiation group were respectively significantly lower than those of the control group and mere irradiated group (t=3.78, 5.79、3.15, P<0.05), however, there was not significant difference between the mere irradiation and control groups(t=2.73, 2.38,P>0.05).Conclusions Celecoxib enhances concentration-dependently the radiosensitivity of human lung carcinoma cell and inhibits the secretion of MMP-2 of the carcinoma cells, thus inhibiting their migration ability. |
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