| JI Rong,CAO Jian-ping,CHEN Xia-lin,et al.Study of radiation sensitization of artesunate on human HeLa cells of cervical cancer[J].Chinese Journal of Radiological Medicine and Protection,2010,30(5):554-557 |
| Study of radiation sensitization of artesunate on human HeLa cells of cervical cancer |
| Received:November 30, 2009 |
| DOI： |
| KeyWords:Radiosensitization Artesunate HeLa cell Apoptosis |
| FundProject:国家自然科学基金(30670632); 教育部长江学者和创新团队发展计划资助项目(IRT0849) |
| Author Name | Affiliation | E-mail | | JI Rong | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | CAO Jian-ping | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | jpcao@suda.edu.cn | | CHEN Xia-lin | 浙江省绍兴市人民医院放疗科 | | | ZHU Wei | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | JIANG Qing | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | PAN Chun-yan | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | ZHOU Yuan-yuan | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | FENG Yang | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | PENG Xiao-mei | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | LIU Yang | School of Radiation Medicine and Public Health,Medical College of Soochow University,Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection,Suzhou 215123,China. | | | FAN Sai-jun | | |
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| Abstract:: |
| Objective To investigate the radiosensitizing effects of artesunate on human HeLa cells of cervical cancer in vitro. Methods Hela cells were irradiated with 60Co γ-rays. The dose rate was 0.635 Gy/min and the radiation dose was 0,1,2,4,6 Gy, respectively. The anti-proliferation activities of artesunate on HeLa cells were evaluated with MTT assay, to determine the most appropriate drug concentration. The effect of radiosensitivity was observed by using clonogenic assay. The single-hit multi-target model was used to plot the HeLa cell’s dose-survival curve, to calculate mean lethal dose, quasi-threshold dose and sensitization enhancement rate, and to evaluate its radiosensitization effect. The apoptosis was analyzed with flow cytometry (FCM) to further test the radiation senseitization of artesunate on HeLa cells. Results The inhibition of artesunate on HeLa cells increased with concentration. In radiation group, the cell cloning efficiency were 91.67%, 82.02%, 58.60%, 25.01%, respectively, and in artesunate (2.0 μmol/L) +radiation group, the cell cloning efficiency were 74.93%, 60.53%, 22.38%, 5.05%. In radiation group and artesunate (2.0 μmol/L) +radiation group, the mean lethal dose(D0 was 2.95 and 2.07 Gy, respectively, while the qusai-threshold dose (D</em>q) were 2.01 and 1.24 Gy,respectively, and SER was 1.43. Compared with 2 and 6 Gy radiation group, the apoptosis rate of drug + radiation group increased from 12.26%, 40.08% to 22.71%, 59.92%. Conclusions The inhibiting effect of artesunate on HeLa cells is concentration-dependent. Artesunate has radiosensitizing effect on HeLa cells in vitro. |
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