| PAN Lu-yu,CAO Jian-ping,JI Rong,et al.Effect of artemether on radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1[J].Chinese Journal of Radiological Medicine and Protection,2010,30(5):550-553 |
| Effect of artemether on radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1 |
| Received:November 28, 2009 |
| DOI： |
| KeyWords:Artemether Nasopharyngeal neoplasms Radiosensitivity |
| FundProject:国家自然科学基金(30670632);教育部长江学者创新团队项目(IRT0849) |
| Author Name | Affiliation | E-mail | | PAN Lu-yu | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | | | CAO Jian-ping | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | jpcao@sudu.edu.cn | | JI Rong | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | | | FENG Yang | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | | | ZHOU Yuan-yuan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | | | PENG Xiao-mei | 江苏省吴江市疾病预防控制中心 | | | LIU Yang | 苏州市市立医院东区 肿瘤内科实验室 | | | CHEN Xia-lin | 绍兴市人民医院 | | | CUI Dan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | | | ZHU Wei | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | | | FAN Sai-jun | School of Radiation Medicine and Public Health, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123,China | |
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| Abstract:: |
| Objective To evaluate the effect of artemether on the cell cycle and the radiosensitivity in human nasopharyngeal carcinoma cell line CNE-1. Methods Cell growth inhibition was assessed with MTT. The method of colony-forming was used to detect the radiation sensitivity. Cell cycle distribution was analyzed by using flow cytometry. The protein expressions of clyclin B1 and Wee1 were detected by using Western blot. Results The growth of CNE-1 cells was inhibited in a dose-dependent manner. The concentration of 20 μmol/L artemether had radiosensitive effect on CNE-1 cells at 24 h after administration, and SER was 1.481. When CNE-1 cell was irradiated, the G2/M cells increased (t=4.59, P<0.05). After exposure to combination of artemether and irradiation, the G2/M cells were decreased (t=10.60, P<0.05). Western blot showed that artemether increased the level of cyclin B1 expression and inhibited the level of Wee1 expression. Conclusions The noncytotoxic concentration of artemether could enhance radiosensitization of CNE-1 cells. The radiosensitivity enhancement of artemether might depend on the exposure time. The effect is most obvious when radiation is delivered 24 h after expose to artemetherr. The radiosensitizing effect could be related to apoptosis. |
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