| DONG Xiao-rong,DONG Ji-hua,ZHANG Rui-guang,FAN Li,LIU Li,ZHANG Tao,WU Gang.Inhibitory effects of Tanshinone Ⅱ A on radiation-induced inflammatory response in microglia BV-2 cells[J].Chinese Journal of Radiological Medicine and Protection,2010,30(5):535-539 |
| Inhibitory effects of Tanshinone Ⅱ A on radiation-induced inflammatory response in microglia BV-2 cells |
| Received:November 24, 2009 |
| DOI： |
| KeyWords:Irradiation DNA double-strand break Microglia cells Inflammatory response Tanshinone ⅡA |
| FundProject:国家自然科学基金(30800283) |
| Author Name | Affiliation | E-mail | | DONG Xiao-rong | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China | | | DONG Ji-hua | 华中科技大学同济医学院附属协和医院肿瘤研究中心, 中心实验室 | | | ZHANG Rui-guang | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China | | | FAN Li | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China | | | LIU Li | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China | | | ZHANG Tao | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China | | | WU Gang | Cancer Center, Union Hospital, Huazhong University of Science and Technology, Wuhan 430022, China | hustxiehe@hotmail.com |
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| Abstract:: |
| Objective To explore the inhibitory effects of Tanshinone ⅡA on the radiation-induced microglia activation and the possible mechanism. Methods Microglia cells BV-2 were irradiated with 2, 4, 8, 16, and 32 Gy doses or sham-irradiated in presence or absence of 1.0 μg/ml Tanshinone ⅡA for 12 h,respectively. The effects of Tanshinone ⅡA on radiation-induced pro-inflammatory cytokines were evaluated using real-time PCR. The expression level of NF-κB p65 in cytoplasm and nucleus was measured by using Western blot. Immunofluorescence staining and confocal microscopy analysis were applied to detect the expression of γ-H2AX and p65 post-irradiation. Results The microglia cells were activated at 16, 32 Gy post-irradiation. Radiation-induced release of the pro-inflammatory cytokines in BV-2 cells was detectable after irradiation. Tanshinone ⅡA decreased radiation-induced release of pro-inflammatory cytokines(t=5.56, P<0.05). Furthermore, western blotting showed that Tanshinone ⅡA could attenuate the nuclear translocation of NF-κB p65 submit post-irradiation. Immunofluorescence staining showed that γ-H2AX foci formation while p65 translocation into nucleus post-irradiation. Conclusions Tanshinone ⅡA exerts anti-inflammatory properties by suppressing the transcription of pro-inflammatory cytokine genes that might be associated with NF-κB signaling pathway. It is postulated that irradiation causes immediate cellular reaction and DSB triggers the molecular response which leads to NF-κB pathway activation. |
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