| SUN Yan,HAN Yun,ZHU Ying-bao.Increased apoptosis of A549 cells by overexpression of helicase-like transcription factor induced by radiation exposure[J].Chinese Journal of Radiological Medicine and Protection,2010,30(5):507-509 |
| Increased apoptosis of A549 cells by overexpression of helicase-like transcription factor induced by radiation exposure |
| Received:April 13, 2010 |
| DOI： |
| KeyWords:Cell apoptosis Helicase-like transcription factor (HLTF) Cytochrome C |
| FundProject:国家自然科学基金(30470976) |
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| Abstract:: |
| Objective To study the effects of overexpression of helicase-like transcription factor (HLTF) on radiation-induced apoptosis of human lung carcinoma cells. Methods Human lung carcinoma cellline A549 were cultured, transfected with FLAG-tagged HLTF expression plasmid pcDNA3.1/HLTF-FLAG (HLTF gene transfection group), empty plasmid vector pcDNA3.1 (empty vector group), and mock transfection group,respectively, and then exposed to 60Co γ-ray irradiation. Cell apoptosis was observed by flow cytometry 48 h post-irradiation. Western blotting was used to detect the levels of HLTF in the nucleus and cytoplasm and the release of cytochrome C from mitochondria into cytoplasm. Results The apoptosis rate of the HLTF gene group was (32.2±2.1)%, significantly higher than those of the empty vector group ［(11.4±2.3)%］ and mock transfection group ［(11.1±1.8)%］ (F=101.85,P<0.05). The level of cytochrome C in the cytoplasm of the HLTF gene group was significantly higher than that of the empty plasmid group. Conclusions HLTF protein sensitizes human lung carcinoma cells to radiation-induced apoptosis, partly by promoting the nuclear-cytoplasma transition of HLTF protein and the increased release of cytochrome C. |
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