| FENG Wei,ZHENG Xiao,WANG Yue-zhen,et al.Effect of γ-rays combined with blocking VEGF expression on esophageal cancer xenografts[J].Chinese Journal of Radiological Medicine and Protection,2010,30(4):395-398 |
| Effect of γ-rays combined with blocking VEGF expression on esophageal cancer xenografts |
| Received:July 20, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.005 |
| KeyWords:Esophageal cancer cell TE-1 Vascular endothelial growth factor (VEGF) Xenografts Irradiation |
| FundProject:浙江省医药卫生优秀青年科技人才专项基金(2008QN005);浙江省省医药卫生科学研究基金项目(C2006B011);卫生部科学研究基金-浙江省医药卫生重大科技计划项目 (WKJ2007-2-004) |
| Author Name | Affiliation | | FENG Wei | Department of Radiation Oncology, Cancer Hospital of Zhejiang, Hangzhou 310022, China | | ZHENG Xiao | Department of Radiation Oncology, Cancer Hospital of Zhejiang, Hangzhou 310023, China | | WANG Yue-zhen | Department of Radiation Oncology, Cancer Hospital of Zhejiang, Hangzhou 310024, China | | WANG Zhun | Department of Radiation Oncology, Cancer Hospital of Zhejiang, Hangzhou 310025, China | | ZHU Shu-chai | 河北医科大学第四医院放疗科 |
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| Abstract:: |
| Objective To investigate the effect of blocking VEGF expression on the radiation sensitivity of esophageal cancer cell line TE-1 in vivo.Methods 32 male Balb/c/nu nude mice were randomly divided into four groups, including control group, radiation group, anti-VEGF group, and anti-VEGF + radiation group. The anti-VEGFcDNA cells were subcutaneously injected into the paw pats of mice(2×106/100 μl).The subcutaneous tumors were irradiated with 18 Gy of 60Co γ-rays when the diameter of tumors varied from 0.8 to 1.0 cm. The volume of the tumors was measured before and after irradiation, respectively. The expression level of VEGF mRNA and protein were examined by RT-PCR and Western blotting, respectively. Apoptotic cells were detected by electron micrographs. Results Latent period of the tumor formation of anti-VEGF group was lengthened compared with other groups(t=13.898, P<0.01).The volumes of tumor in anti-VEGF group[(1207.50±97.07)mm3] and anti-VEGF + radiation group [(1057.5±91.50)mm3] were not statistically different post-irradiation (t=1.124,P>0.05), but smaller than those in control group [(5442.50±185.08)mm3] and radiation group [(2922.50±152.773)mm3] with statistical differences(t=9.475-21.238,P<0.01). The expression level of endogenous VEGFmRNA and protein in anti-VEGF group and anti-VEGF + radiation group were statistically different from control group and radiation group (F=387.394, 13.519, P<0.01).Conclusions Antisense VEGF could inhibit the proliferation of esophageal cancer cell in the nude mice. Effect of blocking VEGF expression before irradiation on esophageal cancer xenografts might be limited. |
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