| ZHU Xiao-dong,SU Fang,QU Song,WANG Qi,LI Li,ZHANG Wei.Variabilities of serum proteomic spectra in patients with nasopharyngeal carcinoma before and after radiotherapy[J].Chinese Journal of Radiological Medicine and Protection,2010,30(4):391-394 |
| Variabilities of serum proteomic spectra in patients with nasopharyngeal carcinoma before and after radiotherapy |
| Received:October 29, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.04.004 |
| KeyWords:Nasopharyngeal carcinoma Surface-enhanced laser desorption ionization time-of flight-mass spectrometry Protein biomarker |
| FundProject:吴阶平医学基金会临床科研专项资助基金(320.6750.06080);广西科学研究与技术开发计划课题(桂科攻0719006-2-23);广西研究生教育创新计划资助项目(2007105981002 M33);广西大型仪器协作共用网测试补助项目(533-2007-112;555-2007-134) |
| Author Name | Affiliation | E-mail | | ZHU Xiao-dong | Department of Radiotherapy, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | zhuxiaodong1966@yahoo.com.cn | | SU Fang | Department of Radiotherapy, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | | | QU Song | Department of Radiotherapy, Guangxi Autonomous Regional Cancer Hospital & Cancer Hospital of Guangxi Medical University, Nanning 530021, China | | | WANG Qi | 530021 南宁, 广西壮族自治区肿瘤医院 广西医科大学附属肿瘤医院放射妇瘤科 | | | LI Li | 530021 南宁, 广西壮族自治区肿瘤医院 广西医科大学附属肿瘤医院放射妇瘤科 | | | ZHANG Wei | 530021 南宁, 广西壮族自治区肿瘤医院 广西医科大学附属肿瘤医院放射妇瘤科 | |
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| Abstract:: |
| Objective To study the changes of serum proteomic spectra in patients with nasopharyngeal carcinoma(NPC) before and after treatment in order to detect the protein biomarkers. Methods Proteomic spectra from serum of 50 NPC patients before radiotherapy,25 NPC patients who achieved complete remission(CR) after radiotherapy, and 40 persons from normal control subjects were analyzed by CM-10 protein chip and surface-enhanced laser desorption ionization time of flight mass spectrometry. Results Expressed proteins in serum were screened by analysis of the proteomic spectra of pre-radiotherapy patients and normal individuals. 4 kinds of proteins with the relative molecular masses of 2931,4098,5343,13766 made up markers pattern which was able to classify the patients and normal individuals. The sensitivity and specificity results were 90.0% and 90.0%, respectively. The twenty differential expression protein peaks of patients before and after radiotherapy were obviously different. The relative molecular masses of 2931, 4182, 4688 and 13766 were up-regulated in untreated NPC, while were close to the normal levels in CR group. Two other protein peaks of 4098 and 5343 were down-regulated in untreated NPC group, which were close to normal levels in CR group.Conclusions The expressions of protein levels are different before and after radiotherapy in NPC patients. Protein signatures of NPC may be screened using SELDI-TOF-MS. Those signatures may be helpful in assessing the minimal residual disease and predicting the treatment efficacy. |
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