| LI Hai-ying,ZHANG Li,WU Jian-bo,et al.Cetuximab enhances the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):291-294 |
| Cetuximab enhances the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R |
| Received:July 13, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.016 |
| KeyWords:Esophageal carcinoma Radiosensitivity Cetuximab |
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| Abstract:: |
| Objective To determine the effect of cetuximab (C225) on the radioresistant human esophageal squamous carcinoma cell line KYSE-150R.Methods A radioresistant human esophageal squamous carcinoma cell line KYSE-150R was established by fractionated irradiation. Morphological changes from KYSE-150 to KYSE-150R were observed by phase-contrast microscopy. Karyotype analysis was performed by G-banding. The radiosensitivities were analyzed by colony formation assays.Results The population doubling time of KYSE-150 and KYSE-150R were (23.6±0.2) h and (25.9±0.6) h(t=6.6,P＜0.01), respectively. The chromosome number of KYSE-150R was increased and chromosome aberrations were observed from (69.3±1.9) h to (73.7±1.2)h (t=-8.83,P<0.01). The SF2, D0, Dq and N values of KYSE-150R were all higher than those of KYSE-150. After 5 μg/ml of C225 added, the SF2, D0, Dq and N values were significantly decreased as compared to the control. After C225 treatment, the G0/G1 and G2/M phase cells were increased,while S-phase cells decreased(t=-4.478-4.308, P<0.05).Conclusion Cetuximab can enhance the radiosensitivity of radioresistant human esophageal squamous carcinoma cell line KYSE-150R. |
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