| TANG Yan,CAO Jian-ping,FAN Sai-jun,ZHU Wei,ZHANG Xu-guang,CHEN Xia-lin,JI Rong,PAN Chun-yan,ZHOU Yuan-yuan,FENG Yang,JIANG Qing.Radiosensitizing effects of artemisinin on CNE human nasopharyngeal carcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):280-282 |
| Radiosensitizing effects of artemisinin on CNE human nasopharyngeal carcinoma cells |
| Received:July 03, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.013 |
| KeyWords:Artemisinin Nasopharyngeal carcinoma cells Radiosensitization Cell cycle |
| FundProject:国家自然科学基金(30670632);长江学者和创新团队发展计划资助(IRT0849) |
| Author Name | Affiliation | E-mail | | TANG Yan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | CAO Jian-ping | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | jpcao@suda.edu.cn | | FAN Sai-jun | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | ZHU Wei | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | ZHANG Xu-guang | 徐州市肿瘤医院 | | | CHEN Xia-lin | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | JI Rong | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | PAN Chun-yan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | ZHOU Yuan-yuan | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | FENG Yang | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | | | JIANG Qing | School of Radiation Medicine and Public Health, Medical College of Soochow University, Suzhou 215123, China | |
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| Abstract:: |
| Objective To investigate the radiosensitizing effects of artemisinin on CNE human nasopharyngeal carcinoma cells in vitro. Methods CNE human nasopharyngeal carcinoma cell line was used in this study. Cell growth kinetics was determined by MTT assay. Effect of the drug on radiosensitivity of CNE cells was analyzed by clonogenic assay. The change of cell cycle was measured by flow cytometry.Results The inhibition of CNE cells growth by artemisinin was increased with concentrations. Artemisinin(1 μmol/L) could enhance the radiosensitizing effects on CNE cell line, and the sensitizing enhancement ratio(SER) was 1.26. Artemisinin abrogated radiation-induced G2/M arrest of the tested CNE cells. Compared with the radiation alone group, the proportion of G2/M phase cells increased in radiation combined with drug group. Conclusions Artemisinin could reduce radiation-induced G2/M arrest and enhance the cytotoxicity of γ-irradiation on the CNE cells. |
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