| YU Jing-ping,SUN Su-ping,SUN Zhi-qiang,et al.Inhibition of DNA synthesis and radiosensization effects of thalidomide on esophageal carcinoma TE1 cells[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):270-273 |
| Inhibition of DNA synthesis and radiosensization effects of thalidomide on esophageal carcinoma TE1 cells |
| Received:November 03, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.010 |
| KeyWords:Thalidomide TE1 cell DNA synthesis Radiosensitivity |
| FundProject:国家自然科学基金(30870585);常州市青年科技培养计划(CQ2008006);常州市社会发展指导性项目(CS2008920) |
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| Abstract:: |
| Objective To explore the radiosensitization effect of thalidomide combined with X-ray on esophageal carcinoma TE1 cells. Methods Cell scratch assay was used to detect the inhibition ability of different concentration of Thalidomide on cell invasion and metastasis. H3-TdR incorporation assay was used to investigate the inhibition of DNA synthesis in TE1 cells by treated with Thalidomide singly or combination with X-rays. The colony formation assay was used to analyze the radiosensitization of Thalidomide effect on TE1 cells. Results Thalidomide had obvious inhibition effect on TE1 cell metastasis, DNA synthesis and colony formation, which were correlated with drug concentration. The values D0, Dq and SF2 in TE1 cells were gradually decreased with thalidomide concentration increased. When the concentration of thalidomide was 100μg/ml, the SERD0 and SERDq were (1.4±0.2) and (1.5±0.1), respectively, While the concentration of thalidomide was 150 μg/ml, the SERD0 and SERDq were (1.5±0.2) and (1.8±0.2), respectively. Conclusions Thalidomide could inhibit TE1 cell invasion, metastasis, DNA synthesis, and significantly enhance the radiosensitizing effect on esophageal carcinoma TE1 cells. |
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