| JIA Li-li,ZHANG Tao,YANG Yong-hua,WANG Yan,DU Li-qing,CAO Jia,WANG Hong,LIU Qiang,FAN Fei-yue.Assessment of DNA damage in radiation workers by using single cell gel electrophoresis[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):267-269 |
| Assessment of DNA damage in radiation workers by using single cell gel electrophoresis |
| Received:December 16, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.009 |
| KeyWords:Ionizing radiation Radiation workers DNA damage Single cell gel electrophoresis |
| FundProject:国家自然科学基金项目(30800281);天津市自然科学基金(07JCYBJC09200) |
| Author Name | Affiliation | E-mail | | JIA Li-li | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | ZHANG Tao | 天津市骨科医院 | | | YANG Yong-hua | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | WANG Yan | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | DU Li-qing | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | CAO Jia | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | WANG Hong | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | LIU Qiang | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | dr.qiangliu@yahoo.com.cn | | FAN Fei-yue | Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College. Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | |
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| Abstract:: |
| Objective To assess the DNA damage of radiation workers in different grade hospitals, and to explore the correlation between the types of work or work time and the levels of DNA damage. Methods DNA single strand break were detected by using alkaline single cell gel electrophoresis(SCGE), and the comet was analyzed with CASP(Comet Assay Software Project). TDNA%, TL, TM and OTM were calculated. Results The parameters of SCGE in the radiation group were higher than those of control group (F=3.93,P<0.01). The significant difference was found not only among the different types of work or different work time, but also among the different grade hospitals(F=1.83,1.91,P <0.05). Conclusions Various levels of DNA damage could be detected in the radiation workers of the two hospitals. DNA damage of radiation workers is less serious in the higher-grade hospital than the lower grade one.Different types of work or work time might affect the DNA damage level. |
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