| XIE Ling,LI Ming,XING Shuang,et al.Initial research of screening for the differentially expressed proteins in beagles irradiated with 4.5 Gy 60Co γ-rays by two-dimensional gel electrophoresis and mass spectrometry[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):252-255 |
| Initial research of screening for the differentially expressed proteins in beagles irradiated with 4.5 Gy 60Co γ-rays by two-dimensional gel electrophoresis and mass spectrometry |
| Received:December 24, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.005 |
| KeyWords:Acute radiation injuries 2-dimensional electrophoresis High definition mass spectrometry(HD-MS) Serum amyloid A |
| FundProject:国防科工委"十一五"核科学基础研究重点项目(A3620060130) |
| Author Name | Affiliation | E-mail | | XIE Ling | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | LI Ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XING Shuang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | HUANG Hai-xiao | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | OU Hong-ling | 第二炮兵总医院 | | | XIONG Guo-lin | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHAO Yan-fang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHAO Zhen-hu | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Ning | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Jin-xiang | 苏州大学医学部 | | | MIAO Jing-cheng | 苏州大学医学部 | | | ZHU Nan-kang | 苏州大学医学部 | | | ZHANG Xue-guang | 苏州大学医学部 | | | CONG Yu-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | LUO Qing-liang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | luoqingliang52@ yahoo. com. cn |
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| Abstract:: |
| Objective To explore the mechanisms of cytokines on acute radiation disease in irradiated beagles. Methods The sera of beagles irradiated with 4.5 Gy γ-rays with cytokines treatment was collected at different time points post irradiation. The two-dimensional gel electrophoresis (2-DE) was used to isolate and compare the differentially expressed proteins in sera. HD-MS was used to analyze the differentially expressed proteins with significance, and the amino acid sequences should be determined. Results High resolution 2-DE gel map was obtained. There were six differentially expressed proteins in sera of irradiated beagles at different time points. Four protein spots were successfully identified by MS. A significant spot was identified as serum amyloid A (SAA) by HD-MS, with relative molecular mass of 13077 and isoelectric point of 6.26.Expression of SAA was not found 1 d pre-irradiation and 36 d post-irradiation, but increased slightly 1 d (0.2166) and significantly 14 d post-irradiation(0.4577). Conclusions The expression of serum amyloid A was consistent with the process of acute radiation injury, which might indicate the turnover of the disease. |
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