| ZHAO Jian-zhi,LI Ming,XING Shuang,HU Zhi-qing,XIONG Guo-lin,XIE Ling,OU Hong-ling,HUANG Hai-xiao,ZHAO Zhen-hu,WANG Ning,WANG Jin-xiang,MIAO Jing-cheng,ZHU Nan-kang,ZHANG Xue-guang,CONG Yu-wen,ZHANG Ri,LUO Qing-liang.Therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):247-251 |
| Therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles |
| Received:December 24, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.004 |
| KeyWords:rhG-CSF rhIL-11 rhIL-2 Hematopoietic injuries Beagles |
| FundProject:国防科工委"十一五"核科学基础研究重点项目(A3620060130) |
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| Abstract:: |
| Objective To observe the therapeutic effects of combined cytokines on hematopoietic injuries induced by 4.5 Gy 60Co γ-rays irradiation in beagles, and to provide experimental evidences for the clinical treatment of extremely severe myeloid acute radiation sickness (ARS). Methods 16 beagles were given 4.5 Gy 60Co γ-rays total body irradiation, and then randomly assigned into irradiation control group, supportive care group and cytokines group. In addition to supportive care, recombinant human granulocyte colony-stimulating factor (rhG-CSF), recombinant human interleukin-11 (rhIL-11) and recombinant human interleukin-2 (rhIL-2) were administered subcutaneouly to dogs in cytokines group. Peripheral blood hemogram was examined once every two days. Bone marrow and peripheral blood were collected to proceed colony cultivation 4 d pre-irradiation and 1 and 45 d post-irradiation. Conventional histopathological sections of sternum were prepared to observe the histomorphology changes. Results After irradiation, the population of all kinds of cells in peripheral blood declined sharply. WBC nadir was elevated (1.04?109/L, but 0.28?109/L and 0.68?109/L for the irradiation control group and the supportive care group separately), the duration of thrombocytopenia was shortened (24 days, but 33 days for the supportive care groug) and red blood cell counts were maintained in the range of normal values after cytokines treatment in combination. The colony forming efficiency of haemopoietic stem cells (HSCs) in bone marrow and peripheral blood decreased obviously 1 d post irradiation, but recovered to the level of that before irradiation 45 d post irradiation after supportive care and cytokines treatment. Hematopoietic cells disappeared in bone marrow of animals in irradiation control group, but hematopoietic functions were recovered after cytokines were administrated. Conclusions RhG-CSF, rhIL-11 and rhIL-2 used in combination could elevate WBC nadir, accelerate the recovery of leukocytes, platelets and red blood cells and promote the proliferation, differentiation and maturity of HSPCs left in the body after 4.5 Gy γ-rays total body irradiation, eventually restore the hematopoietic function. Hence, combination of rhG-CSF, rhIL-11 and rhIL-2 could serve as better therapeutic strategy to treat extremely severe myeloid ARS. |
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