| LI Ming,OU Hong-ling,XING Shuang,et al.Studies on mechanism of treatment of granulocyte colony-stimulating factor, recombinant human interleukin-11 and recombinant human interleukin-2 on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles[J].Chinese Journal of Radiological Medicine and Protection,2010,30(3):239-242 |
| Studies on mechanism of treatment of granulocyte colony-stimulating factor, recombinant human interleukin-11 and recombinant human interleukin-2 on hematopoietic injuries induced by 4.5 Gy γ-rays irradiation in beagles |
| Received:December 24, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2010.03.002 |
| KeyWords:Acute radiation sickness Cytokines Hematopoietic injuries CD34 cells Cell cycle Apoptosis Beagles |
| FundProject:国防科工委"十一五"核科学基础研究重点项目(A3620060130) |
| Author Name | Affiliation | E-mail | | LI Ming | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | OU Hong-ling | 第二炮兵总医院 | | | XING Shuang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | HUANG Hai-xiao | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XIONG Guo-lin | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XIE Ling | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHAO Yan-fang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHAO Zhen-hu | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Ning | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Jin-xiang | 苏州大学医学部 | | | MIAO Jing-cheng | 苏州大学医学部 | | | ZHU Nan-kang | 苏州大学医学部 | | | LUO Qing-liang | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | CONG Yu-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | ZHANG Xue-guang | 苏州大学医学部 | xgzh@ yahoo. com. cn |
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| Abstract:: |
| Objective To investigate the mechanism of treatment of granulocyte colony-stimulating factor (rhG-CSF), recombinant human interleukin-11 (rhIL-11) and recombinant human interleukin-2 (rhIL-2) on hematopoietic injuries induced by 4.5 Gy 60Co γ-ray irradiation in beagles, and to provide experimental evidence for the clinical treatment of extremely severe myeloid acute radiation sickness (ARS). Methods Sixteen beagle dogs were given 4.5 Gy 60Co γ-ray total body irradiation (TBI), then randomly assigned into irradiation control group, supportive care group or cytokines supportive care (abbreviated as cytokines) group. In addition to supportive care, rhG-CSF, rhIL-11 and rhIL-2 were administered subcutaneously to treat dogs in cytokines group. The percentage of CD34 cells, cell cycle and apoptosis of nucleated cells in peripheral blood were examined by Flow cytometry. Results After 4.5 Gy 60Co γ-ray irradiation, the CD34 cells in peripheral blood declined obviously (61.3% and 52.1% of baseline for irradiation control and supportive care group separately). The cell proportion of nucleated cells in G0/G1 phase was increased notably (99.27% and 99.49% respectively). The rate of apoptosis (26.93% and 21.29% separately) and necrosis (3.27% and 4.14% , respectively) of nucleated cells were elevated significantly when compared with values before irradiation (P<0.05) 1 d post irradiation. When beagles were treated with cytokines and supportive care, the CD34 cells in peripheral blood were markedly increased (135.6% of baseline). The effect of G0/G1 phase blockage of nucleated cells became more serious (99.71%). The rate of apoptosis (5.66%) and necrosis (1.60%) of nucleated cells were significantly lower than that of irradiation control and supportive care groups 1 d after exposure. Conclusions Cytokines maybe mobilize CD34 cells in bone marrow to peripheral blood, indce cell cycle block at G0/G1 phase and reduce apoptosis, and eventually cure hematopoietic injuries induced by irradiation. |
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