| LIU Jun,LUO Qiong,CUI Xiao-yan,et al.Study of the intervention effect of Laminaria japonica polysaccharides on testis tissue in male rats exposed by repeated low-dose ionizing radiation[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):173-176 |
| Study of the intervention effect of Laminaria japonica polysaccharides on testis tissue in male rats exposed by repeated low-dose ionizing radiation |
| Received:July 27, 2009 |
| DOI： |
| KeyWords:Laminaria japonica polysaccharides Ionizing radiation Oxidative damage Rat Testis |
| FundProject:达能膳食营养与宣教基金(DIC2007-06) |
| Author Name | Affiliation | E-mail | | LIU Jun | School of Public Health, Wuhan University, Wuhan 430071,China | | | LUO Qiong | School of Public Health, Wuhan University, Wuhan 430071,China | drluoqiong@sina.com | | CUI Xiao-yan | School of Public Health, Wuhan University, Wuhan 430071,China | | | YANG Ming-liang | School of Public Health, Wuhan University, Wuhan 430071,China | | | YAN Jun | School of Public Health, Wuhan University, Wuhan 430071,China | |
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| Abstract:: |
| Objective To investigate the effect of the Laminaria japonica polysaccharides(LJP) intervention on the male rats’testicular tissue oxidative damage which was caused by repeated low-dose local ionizing radiation. Methods Male Wistar rats were randomly divided into control group, model group and LJP intervention group. The rats in the LJP intervention group was given with LJP. The rats in each group were exposed to 60Co γ-ray local irradiation 7 d after adaptability breeding, once per day for 20 times in 4 weeks. Each rat was irradiated with the total dose of 2.3 Gy. The rats were killed at 1, 7 and 14 d post-irradiation, respectively. The testis and the epididymis were taken out. The contents of MDA and GSH, and the activities of SOD, LDH and GSH-Px were measured using spectrophotometer. The amorphous of testicular tissue was observed and the sperm count and viability were analyzed. Results As compared with those in the model group, the content of MDA decreased in testicular tissue in the LJP group(t=3.66-5.03, P<0.01), while the GSH content increased. The activities of SOD, LDH and GSH-Px(t=2.77-25.52, P<0.05) and the sperm count and viability increased(t=3.11-23.08,P<0.01). Each index was more close to that in the control group 14 d post-irradiation.Conclusions LJP can promote the recovery of testicular tissue oxidative damage caused by chronic local ionizing radiation. It has a role in promoting the spermatogenic function of male rats. |
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