| WANG Zhi-dong,GE Chang-hui,XU Wang-xiang,ZHAN Yi-qun,TANG Li-xuan,CAO Meng-meng,YANG Xiao-ming.Expression of CBLB502 in prokaryocytes and verification of radiation protective effect[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):169-172 |
| Expression of CBLB502 in prokaryocytes and verification of radiation protective effect |
| Received:August 21, 2009 |
| DOI： |
| KeyWords:Toll-like receptor 5 CBLB502 protein Radiation protection |
| FundProject: |
| Author Name | Affiliation | E-mail | | WANG Zhi-dong | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | | | GE Chang-hui | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | | | XU Wang-xiang | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | | | ZHAN Yi-qun | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | | | TANG Li-xuan | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | | | CAO Meng-meng | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | | | YANG Xiao-ming | Beijing Institute of Radiation Mediation Medicine, Beijing 100850, China | xiaomingyang@sina.com |
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| Abstract:: |
| Objective To obtain the purified CBLB502 protein as an agonist of Toll like receptor 5 in prokaryocytes and to validate its radiation protective effect. Methods pET28b expression system was used to express CBLB502 protein, and the Ni-NTA agarose and purification column was used to purify CBLB502 protein. The radiation proective effect of CBLB502 was evaluated by NF-κB reporter gene assay and mice experiments. Results CBLB502 protein was expressed and purified, which could activate NF-κB reporter gene in vitro and improved the livability of irradiated mice by 9 Gy γ-ray.Conclusions CBLB502 protein could be successfully expressed and purified using prokaryotic expression system. It has the excellent radiation protective effect in mice. |
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