| ZHANG Ping,WANG Ping,HAN Lin,et al.Changes of nm23-H1 gene expression in human peripheral blood cell after X-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):161-164 |
| Changes of nm23-H1 gene expression in human peripheral blood cell after X-ray irradiation |
| Received:August 28, 2009 |
| DOI： |
| KeyWords:X-rays nm23-H1 Gene expression changes Biodosimeter |
| FundProject:河南省医学科技创新项目(2005077) |
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| Abstract:: |
| Objective To investigate the expression change of nm23-H1 gene in human peripheral blood cells exposed to ionizing irradiation, and explore the feasibility of nm23-H1 gene expression changes as a biodosimeter. Methods Human peripheral whole blood samples were collected from four healthy individuals, and exposed to X-rays with the doses between 0 and 5 Gy. The total mRNAs were isolated from the white-blood cells, and the changes of nm23-H1 gene mRNA expression post-irradiation were detected by real-time RT-PCR at 0, 6, 12 and 24 h, respectively. Results The levels of nm23-H1 gene expression in human peripheral blood cells increased gradually with the doses from 1 to 3 Gy at 6 h (F=15.064, P<0.05), and from 1 to 5 Gy at 12 h (F=3.435, P<0.05) after irradiation. However, gene expression levels at 0 and 24 h were not significantly different between various groups(F=0.478,0.444, P> 0.05). The time-effect curve showed that the expression of nm23-H1 gene decreased with the doses of 0, 4 and 5 Gy after irradiation (F=8.636,4.112,5.411, P<0.05). Meanwhile, the expression of nm23-H1 gene showed the difference only at 12 h with 1 Gy irradiation(t =-2.527, P<0.05)and was the lowest at 24 h with 2 and 3 Gy irradiation (F =12.517,6.622,P<0.05). Conclusions The expression change of nm23-H1 gene could be induced by X-ray in human peripheral blood cells, and might be a potential biodosimeter candidate applicable in early-response radiation accident victims. |
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