| LI Xin-chun,SONG Liang-wen,YANG Lei-lei,et al.Effects of pirfenidone antagonizing pulmonary fibroblasts transformation caused by radiation[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):156-160 |
| Effects of pirfenidone antagonizing pulmonary fibroblasts transformation caused by radiation |
| Received:January 19, 2009 |
| DOI： |
| KeyWords:Pirfenidone Radiational pulmonary injury Fibroblasts Transformation |
| FundProject:国家自然科学基金(30570545) |
| Author Name | Affiliation | E-mail | | LI Xin-chun | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | SONG Liang-wen | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | Lsong1@yahoo.com.cn | | YANG Lei-lei | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Shao-xia | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | DIAO Rui-ying | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | WANG Xiao-min | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | | | XU Xin-ping | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China | |
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| Abstract:: |
| Objective To explore the effects of pirfenidone antagonizing pulmonary fibroblasts (Fb) transformation towards myofibroblasts (MFb) during the course of radiational pulmonary injury. Methods Wistar rats were randomly assigned into three groups: normal control group, radiation group and radiation and administration group.The rats were given with pirfenidone by intragastric administration 2 days before radiation. The whole breasts of rats were irradiated by 60Co γ-rays at a dose of 20 Gy and 2 weeks after radiation area density of pulmonary radiational inflammation was determined by image analysis. The ratio of pulmonary humid weight and dry weight was calculated. α-smooth muscle actin (α-SMA) expression of pulmonary fibroblasts was examined by immunohistochemistry. Human lung fibroblasts (HLF) abnormal proliferation and TGF-β1 expression were respectively detected by MTT colorimetric method and immunocytochemical stain. Results Compared with radiation group, the area density of pulmonary inflammatory pathological changes, the ratio of pulmonary humid weight and dry weight and pulmonary α-SMA content in rats of radiation and administration group were reduced by 14.0%, 3.82% and 52.8%, respectively. Pirfenidone could suppress radiation-enhanced HLF proliferation and TGF-β1 expression in a dose-dependent manner. Conclusions Pirfenidone can antagonize the induction of interstitial pneumonia in the earlier period of radiational pulmonary injury evidently, and it can significantly suppress HLF abnormal proliferation, TGF-β1 expression and transformation towards MFb, which help for preventing and curing radiational pulmonary fibrosis effectively. |
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