| XIONG Jie,ZHOU Yun-feng,SUN Wen-jie,et al.Study of cancer-specific chimeric promoters induced by irradiation[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):152-155 |
| Study of cancer-specific chimeric promoters induced by irradiation |
| Received:June 28, 2009 |
| DOI： |
| KeyWords:Human telomerase reverse transcriptase gene promoter CArG elements Gene therapy |
| FundProject:国家自然科学基金(30672438);湖北省创新群体项目(2006ABC009) |
| Author Name | Affiliation | E-mail | | XIONG Jie | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | | | ZHOU Yun-feng | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | yfzhouwhu@163.com | | SUN Wen-jie | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | | | WANG Wei-feng | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | | | LIAO Zheng-kai | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | | | ZHOU Fu-xiang | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | | | XIE Cong-hua | Department of Radiochemotherapy, Zhongnan Hospital, Wuhan University, Hubei Key Laboratory of Tumor Biological Behaviors, Hubei Cancer Clinical Study Center, Wuhan 430071, China | |
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| Abstract:: |
| Objective To combine the radio-inducible CArG element with cancer-specific human telomerase reverse transcriptase(hTERT) gene promoter, and to construct the novel chimeric promoters. Methods The synthetic hTERT promoters containing different number of radio-inducible CArG elements were constructed, and the activities of the promoters in the cancer cells (HeLa, A549, and MHCC97 cells) and nomal cells(hEL cells) were detected by using luciferase-reporter assays afer the treatment of irradiation(a single or fractionated irradiation dose). Results Synthetic promoter containing 6 repeated CArG units was better in radio-inducibility than any other promoters containing different number of CArG units, and nearly maximum levels obtained at 4-6 Gy. The very low activities of the chimeric promoters could be detected in normal hEL cells. A similar level of reporter gene expression was observed after 3 fractionated doses of 2 Gy compared with a single dose of 6 Gy in cancer cells. Conclusions The cancer-specific chimeric promoter containing 6 CArG elements showes the best radio-response, and the chimeric promoter system has the potential in cancer gene therapy. |
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