| WU Ning,LIU Li-bo,ZHANG Xuan,et al.Quantitative detection of Egr-1 and Egr-4 gene expression in mice thymus after whole-body irradiation with X-rays by real-time RT-PCR[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):147-151 |
| Quantitative detection of Egr-1 and Egr-4 gene expression in mice thymus after whole-body irradiation with X-rays by real-time RT-PCR |
| Received:March 10, 2009 |
| DOI： |
| KeyWords:Real-time quantitative RT-PCR Egr-1 Egr-4 Bone marrow polychromatic erythrocyte micronucleus Radiation biodosimeter |
| FundProject:卫生部卫生行业科研专项基金(200802018) |
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| Abstract:: |
| Objective To investigate the changes of Egr-1 and Egr-4 gene expression in mice thymus after whole-body irradiation(WBI)with X-rays by real-time quantitative reverse transcription-polymerase chain reaction(QRT-PCR). Methods The mRNA was isolated from mice thymus 4 and 24 h after WBI with 0-6 Gy of X-ray irradiation. The changes of Egr-1 and Egr-4 gene expression 4 and 24 h post irradiation were examined with QRT-PCR. Parallelled counts of micronucleus rate in bone marrow polychromatic erythrocytes(PCE)was made as a reference radiation biodosimetric control.Results The relative expression of Egr-1 and Egr-4 genes at 4 and 24 h after WBI with 0.5 to 6 Gy was changed with the dose (r =0.974,0.987,0.978,0.999, P＜0.01).At all dose points the relative expression of Egr-1 and Egr-4 genes was highly correlated with the micronucleus rate of bone marrow PCE(r =0.866,0.947,0.983,0.835, P＜0.05). The dose-effect relationship could be fitted into linear-quadratic model. The expression of Egr-4 gene was significantly increased at 4 h post irradiation and best correlated with PCE micronucleus rate. Conclusions QRT-PCR assay of early expression of Egr-4 gene might be a candidate for fast, high-throughput radiation biodosimetry. |
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