| SUN Bin,YANG Zhan-shan,ZHOU Zheng-yu,JIN Mei-fang.Experimental study of treatment for radiation-damaged mice by transgenic VEGF[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):138-142 |
| Experimental study of treatment for radiation-damaged mice by transgenic VEGF |
| Received:June 25, 2009 |
| DOI： |
| KeyWords:Gene therapy Vascular endothelial growth factor Radiation damage Mice |
| FundProject:国家自然科学基金(30570549);国防基础科研资助项目(A3820060138) |
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| Abstract:: |
| Objective To study the effect of VEGF gene expression in the treatment of radiation damage, and to explore its molecular mechanism by transferring eukaryotic expression plasmid containing VEGF gene into irradiated mice cells.Methods Normally Kunming mice were divided randomly into three groups as control group, irradiated group and transferred VEGF gene group. The mice were administered with 8 Gy X-ray exposure after intramuscular injection of VEGF recombinant plasmid in the transgenic group. The animals were killed at different times after X-ray exposure. Their clinical manifestation, mortality rate, pathology of tissues and organs and in situ apoptosis in thymus and splenic cells were observed.Results VEGF165 gene fragments were amplified from pSP73/HVEGF165 plasmid by PCR method,and then linked with pcDNA3.1 vector after incision by double enzyme. The recombinant plasmid pcDNA3.1/VEGF165 was constructed. Electrophoresis and sequencing showed that the recombinant plasmid sequence was exactly the same with the data in GenBank. The mortality of irradiated group and transgenic group 14 d post-irradiation was 64％ and 36%, respectively, with the statistical difference (t=3.92, P<0.05). Compared with irradiated group, the apoptosis rate in thymus and spleen cells in transgenic group were decreased significantly (t=3.11,3.53, P<0.05). The radiation-induced pathologic damage was obviously attenuated. Conclusions The recombinant plasmid pcDNA3.1/ VEGF165 was successfully constructed. Transgenic treatment with recombinant plasmid can remarkably decrease the mortality and apoptosis rate of thymus and spleen cells in mice suffering from severe radiation damage, and improve the pathologic change of immune organs. VEGF transgenic technique is one of the effective methods for treating severe radiation injury. |
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