| GAO lin,LIU Xiao-dong,MA Shu-mei.Changes of beclin 1 expression in radiation-induced autophagy in breast cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2010,30(2):124-128 |
| Changes of beclin 1 expression in radiation-induced autophagy in breast cancer cells |
| Received:November 03, 2009 |
| DOI： |
| KeyWords:Autophagy X-rays MAP1LC3B beclin 1 |
| FundProject:国家自然科学基金(30770649,30970682) |
| Author Name | Affiliation | E-mail | | GAO lin | Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China | | | LIU Xiao-dong | Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China | | | MA Shu-mei | Key Laboratory of Radiobiology,Ministry of Health,School of Public Health,Jilin University,Changchun 130021,China | shmm2001@yahoo.com.cn |
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| Abstract:: |
| Objective To investigate the changes of beclin 1 expression in radiation-induced autophagy in breast cancer cell line MCF-7.Methods Real-time RT-PCR was used to detect the expression of autophagy-related genes MAP1LC3B.Reverse transcription-PCR was used to detect the expression of beclin 1.Western blot was used to analyze the protein expression of beclin 1.Results Dose-response of MAP1LC3B mRNA studies showed that MAP1LC3B mRNA increased in 8, 16 and 32 h dose-effect studies, up to peak at 12 Gy in 8 h study(F =13.831, P＜0.05), 12 Gy in 16 h study (F =10.996, P＜0.01) and 8 Gy in 32 h study (F =17.019, P＜0.01), respectively. Time-response studies showed that MAP1LC3B mRNA increased and reached to peak at 16 h in 2 Gy dose-effect study (F =16.284, P＜0.01), 32 h in 8 Gy study (F =9.030, P＜0.05) and 16 h in 12 Gy study (F =20.315, P＜0.05), respectively.Dose-response studies of beclin 1 mRNA showed that beclin 1 increased after 2, 4 and 8 Gy irradiation. Time-response studies showed that beclin 1 increased in a time-dependent manner after 8 Gy irradiation.16 h dose-effect study of beclin 1 protein showed the increase of beclin 1 expression, and 2 Gy time-response study showed that beclin 1 expression increased and reached the peak at 8 h.Conclusions X-ray irradiation could induce autophagy in MCF-7 cells. beclin 1 might play an important role in the process of autophagy. |
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