| LIU Jian-xiang,RUAN Jian-lei,TIAN Mei,PAN Yan,SU Xu.Effects of partial radiation in vitro on chromosome aberrations in human peripheral blood lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2010,30(1):26-28 |
| Effects of partial radiation in vitro on chromosome aberrations in human peripheral blood lymphocytes |
| Received:August 28, 2009 |
| DOI： |
| KeyWords:Partial irradiation Human peripheral blood Chromosome aberration |
| FundProject:卫生行业科研专项(200802018) |
| Author Name | Affiliation | E-mail | | LIU Jian-xiang | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | TIAN Mei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | PAN Yan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To analyze the chromosome aberrations induced by partial radiation in human peripheral blood lymphocytes in vitro . Methods Heparinized whole blood samples were exposed to 2 Gy 60Coγ-rays at 37℃, and then mixed with non-irradiated blood by different ratio. The slides were prepared after culturing and the unstable aberrations were analyzed. Results The chromosome aberrations had a good relationship with the ratio of irradiated blood. The chromosome aberrations in partial irradiated group were higher than that in the irradiated group. The estimated dose was 1.27 Gy when the ratio was 1:1, greater than the dose of 1 Gy. The estimated dose was 0.93 Gy when the ratio was 0.5:1, also greater than 0.5 Gy. But when the ratio was 1:0, the radiation dose was accordant with the estimated dose. Conclusions Chromosome aberrations could be a biomarker for estimating the uneven irradiation. |
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