| NIE Qi,LIU Lu,LIU Zhi-yong,HUANG Pei-lin,LAN Xing-hao,GAO Hai-lin,WU Qing-hua,SUN Jin,HUANG Ying.Cumulative damage effect of 32P-colloidal chromic phosphate interstitial delivery on beagles[J].Chinese Journal of Radiological Medicine and Protection,2010,30(1):9-14 |
| Cumulative damage effect of 32P-colloidal chromic phosphate interstitial delivery on beagles |
| Received:June 29, 2009 |
| DOI： |
| KeyWords:32P-colloidal chromic phosphate Interstitial Experimental animal Security |
| FundProject:国家863项目(2007AA02Z471) |
| Author Name | Affiliation | E-mail | | NIE Qi | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | LIU Lu | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | luliuzhou@sina.com | | LIU Zhi-yong | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | HUANG Pei-lin | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | LAN Xing-hao | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | GAO Hai-lin | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | WU Qing-hua | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | SUN Jin | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | | | HUANG Ying | Nuclear Medicine Institute of Southeast University Clinical Medicine, Nanjing 210009, China | |
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| Abstract:: |
| Objective To explore the possibility and safety of 32P-colloidal chromic phosphate interstitial injection. Methods Ten Beagle dogs were randomly divided into 5 groups ( n =2) according to different doses (185 and 370 MBq), different sites (gluteus maximus and liver) and cold colloid as a control group. At different time-points after surgery, the weights of dogs were measured, and the blood and blood biochemical inspections were examined. ECT imaging was performed and histomorphology was observed dynamically. The radioactive counts of body surface for 90 days, blood for 12 weeks and urine and feces for 30 days were measured continuously. Measured data were expressed by mean ± standard error ( ±s) and SPSS 13.0 software was used for statistical analysis. Results ECT imaging demonstrated that the whole liver imaging was obtained although the radioactive distribution was uneven in liver groups, and the radioactivity concentrated continuously in the area of injection, but no liver imaging in muscle groups. Dogs in group 4 lost weight progressively and reduced by 2.7 kg till 45 d after operation. While the mean weight increments in the other groups were 3.0, 1.6, 0.8 and 3.1 kg in order. In group 4, PLT and RBC reduced obviously. Dogs died at 23 or 45 d. AST and ALT were elevated sharply before death. In the other groups, blood and blood biochemistry inspection showed there were no significant statistical differences. The highest radioactive counts after operation were obtained from the injection spot, while the urinary bladder and the spleen were followed. The peak of blood cpm in liver groups presented at 5 min.Peak values were 0.5×107/min and 1.0×107/min, respectively. The blood cpm in the muscle groups was always maintained at 3×105/min. Histology study showed the hyperemia dropsy changes in muscle groups and 185 MBq liver group in 4 weeks, while after 8 weeks the organizational structure restored normally. There were partial liver cells necrosis in 4 weeks, and the massive liver cells balloon type changes in 6 weeks, as well as obvious hyperemia dropsy and the hepatic lobe structure unclear in 370 MBq liver group. For the radioactive counts of urine and excrement, the peak appeared at 13 and 12 d respectively, and the peak values were (42.0±3.3)×104 and (29.6±4.5)×104/min in muscle groups, respectively ; while the peak appeared at 5 and 9 d, respectively and the peak values were (49.0±10.2)×104 and (28.5±7.1)×104/min in liver groups, respectively. Cumulative excretion ratios in urine and excrement were 36.58% and 10.62% in muscle groups, respectively and 23.48% and 8.76% in liver groups till 30 d, respectively. The liver absorbed doses were 30.6 and 55.6 Gy in liver groups, while those were 2.3 and 6.5 Gy in muscle groups. The maximus absorbed doses of gluteus were 53.4 and 98.1 Gy in muscle groups. Conclusions When 32P-colloidal chromic phosphate of 794.39 MBq/m2 was injected into the liver of Beagle, the liver absorbed dose was 56 Gy, which could be lethal dose for its strong liver toxicity and systemic side effects. Injection of 463.98-772.93 MBq/m2 in muscle of Beagle could be safe. 32P-colloidal chromic phosphate interstitial injection is secure to treat the solid tumors with poor and middle blood supply which could be reached by puncture. |
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