| LIU Han-chen,LI Yu,LI Bai-long,CUI Jian-guo,GAO Fu,SUN Ding,SHAN Hu-sheng,ZHAO Fang,HUANG Yue-cheng,DONG Jun-rui,LIU Hao,CAI Jian-ming.Differential radiobiological effects of carboxyfullerene C3on K562 and AHH-1 cells[J].Chinese Journal of Radiological Medicine and Protection,2010,30(1):1-4 |
| Differential radiobiological effects of carboxyfullerene C3on K562 and AHH-1 cells |
| Received:June 26, 2009 |
| DOI： |
| KeyWords:Carboxyfullerene C3 K562 cell AHH-1 cell γ-ray Apoptosis |
| FundProject:国家自然科学基金(30570552);全军医药卫生"十一五"攻关课题(06G60) |
| Author Name | Affiliation | E-mail | | LIU Han-chen | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | LI Yu | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | LI Bai-long | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | CUI Jian-guo | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | GAO Fu | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | SUN Ding | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | SHAN Hu-sheng | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | ZHAO Fang | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | HUANG Yue-cheng | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | DONG Jun-rui | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | LIU Hao | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | | | CAI Jian-ming | Department of Radiation Medicine, Second Military Medical University, Shanghai 200433, China | cjm882003@yahoo.com.cn |
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| Abstract:: |
| Objective To investigate the application prospective of carboxyfullerene C3as a radioprotectant or assistant for tumor radiotherapy. Methods Different concentrations of C3 were incubated with K562 and AHH-1 cell, CCK-8 assay and trypan blue rejection test were performed to examine the influence of C3 on the cell viability. AnnexinV/PI staining and flow cytometry assay were applied to assess the cell cycle and apoptosis after γ-ray irradiation. Results C3 showed little toxicity to AHH-1 cell with the survival rate over 95%, but 600 mg/L of C3 markedly inhibited the growth of K562 cell(82%). Pretreatment of 100 mg/L C3 significantly increased the survival rate of AHH-1 cell after 4 Gy irradiation compared with the single radiation group(71.3%vs 90.3%), but decreased the apoptosis rate(26.3% vs 12.6%), while the survival rate of K562 cell was decreased and the apoptosis rate was elevated with the increase of C3 concentration. Moreover, the cell cycle analysis revealed the G2 phase block in AHH-1 cell after radiation exposure was mitigated by C3 pretreatment, but that in K562 cell was aggravated. Conclusions C3 has good radioprotective effects on AHH-1 cells. For K562 cell, C3 could inhibit the cell proliferation, promote the radiation induced apoptosis and aggravate the G2 phase block. |
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