| YANG Yun-yi,ZHANG Li,WANG Juan,ZHAO Heng-fang,DAI Li,LIU Zi.Effect of the γ-IFN on radiation damage and radiation fibrosis of the rabbit rectum after cavitas pelvisrute fractionated irradiation[J].Chinese Journal of Radiological Medicine and Protection,2009,29(6):595-598 |
| Effect of the γ-IFN on radiation damage and radiation fibrosis of the rabbit rectum after cavitas pelvisrute fractionated irradiation |
| Received:December 27, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.010 |
| KeyWords:γ-IFN Radiation_induced rectum injury Radiation rectum fibrosis TGF-β1 ColⅢ Rabbit |
| FundProject:陕西省科技攻关项目(2005K12-G5-4) |
| Author Name | Affiliation | E-mail | | YANG Yun-yi | Department of Radiation Oncology, The First Hospital of Xi'an Jiaotong University, Xi'an 710061, China | | | ZHANG Li | Department of Radiation Oncology, The First Hospital of Xi'an Jiaotong University, Xi'an 710061, China | | | WANG Juan | Department of Radiation Oncology, The First Hospital of Xi'an Jiaotong University, Xi'an 710061, China | | | ZHAO Heng-fang | Department of Radiation Oncology, The First Hospital of Xi'an Jiaotong University, Xi'an 710061, China | | | DAI Li | Department of Radiation Oncology, The First Hospital of Xi'an Jiaotong University, Xi'an 710061, China | | | LIU Zi | Department of Radiation Oncology, The First Hospital of Xi'an Jiaotong University, Xi'an 710061, China | liuzmail@163.com |
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| Abstract:: |
| Objective To investigate the effect of γ-IFN on the radiation-induced injury and fibrosis of the rabbit rectum after the fractionated irradiation to pelvic cavity. Methods The animal model of radiation rectum injury was established. 52 New Zealand rabbits were divided into 3 groups as the normal control group, the conventional radiation group, and γ-IFN group. Except the normal control group, the pelvic cavity of the two other groups were irradiated with 6 MV X-rays. At 5 d post-irradiatio the γ-IFN group was administered with 250 000 U/kg of γ-IFN once a week, while the conventional radiation group with 5 ml of saline solution. The rabbits were executed after 4,8,12 and 16 weeks, and the recta in the irradiated field were taken out. HE dyeing, in-situ hybridization and immuno-histochemical methods were used to analyze the radiation-induced rectrum injury and fibrosis. Results At 4,8, and 12 weeks post-irradiation, the radiation rectum injury and fibrosis were significantly different between the conventional radiation group and γ-IFN group(P<0.05), but not at 16 weeks (P>0.05). At 4, 8, 12 and 16 weeks post-irradiation, the expression of TGF-β1 mRNA in-situ hybridization in the γ-IFN group were significantly different compared to the conventional radiation group and the normal control group (P<0.05). The immuno-histochemical results of Col Ⅲ showed the significant difference among theγ-IFN group and both the normal control group and conventional radiation group (P<0.05). Conclusions γ-IFN could reduce the radiation rectum injury, suppress the expression of TGF-β1 mRNA in the rectum mucosa, inhibit the expression of Col Ⅲ,and delay the formation of radiation-induced fibrosis. |
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