| PAN Yan,TIAN Mei,LIU Jian-xiang,RUAN Jian-lei,SU Xu.DNA damage and γH2AX expression in EJ cells induced by 60Cogamma-rays[J].Chinese Journal of Radiological Medicine and Protection,2009,29(6):586-589 |
| DNA damage and γH2AX expression in EJ cells induced by 60Cogamma-rays |
| Received:December 19, 2008 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.008 |
| KeyWords:60Coγ-rays Irradiation γH2AX DNA damage |
| FundProject:卫生部卫生行业科研专项(200802018) |
| Author Name | Affiliation | E-mail | | PAN Yan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | TIAN Mei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LIU Jian-xiang | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | RUAN Jian-lei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | SU Xu | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | suxu@nirp.cn |
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| Abstract:: |
| Objective To investigate 60Coγ-rays induced DNA damage of human bladder cancer cell line EJ cells and the relationship between different doses of 60Coγ-rays, γH2AX foci number and γ-H2AX expression level. Methods EJ cells were exposed to different doses of 60Coγ-rays and the oliver tail moment (TM) of EJ cells were analyzed with single cell gel electrophoresis (SCGE). Immunofluorescent microscopy was used to analysis γ-H2AX foci formation in EJ cells after exposure to different doses of γ-ray irradiation and time-course after exposure to 2 Gy γ-ray irradiation. FACSAria was used to detect the changes of γH2AX protein expression in EJ cells. Results The TMs of EJ cells were increased with the irradiation dose. The TM of 0 Gy group and 4 Gy group was 0.24 and 5.26, respectively. Immunofluorescent analysis demonstrated that γH2AX foci could be induced by γ-ray irradiation in dose-dependent and time-dependent manners. The foci number and size in nuclei of EJ cells was significantly increased after exposed to different doses of γ-ray irradiation and the foci remained detectable at 24 h after exposed to irradiation. The dose range in which foci could be clearly detected was from 0.1 to 4 Gy. FACSDiva showed that γ-H2AX protein expression was increased after exposure to different doses of γ-ray irradiation. γH2AX protein expression level of 0.1 Gy group and 4 Gy group was 7.4% and 29.2%, respectively. Conclusions γH2AX foci could be the most sensitive indicator for DNA damage and repair in mammalian cells, and it might be a new biomarker for radiological emergency. |
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