| XU Yang,WANG Jun-ping,CHEN Fang,SHEN Ming-qiang,CHEN Mo,WANG Song,RAN Xin-ze,SU Yong-ping,KAI Li.Effect of rTMP-GH recombinant fusion protein on thrombocytopoiesis in irradiation injured mice[J].Chinese Journal of Radiological Medicine and Protection,2009,29(6):586-585 |
| Effect of rTMP-GH recombinant fusion protein on thrombocytopoiesis in irradiation injured mice |
| Received:February 17, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.007 |
| KeyWords:Thrombopoietin mimetic peptide Growth hormone Irradiation injury Thrombocytopoiesis |
| FundProject:国家863计划(2007AA02Z152);国家教委高校全国优博论文专项资金(200362);新药创制科技专项(2008ZXJ09003-011) |
| Author Name | Affiliation | E-mail | | XU Yang | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | WANG Jun-ping | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | wangjunp@yahoo.com | | CHEN Fang | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | SHEN Ming-qiang | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | CHEN Mo | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | WANG Song | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | RAN Xin-ze | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | SU Yong-ping | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | | | KAI Li | State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Preventive Medicine, Third Military Medical University, Chongqing 400038, China | |
|
| Hits: 2776 |
| Download times: 2013 |
| Abstract:: |
| Objective To investigate the in vivo effects of rTMP-GH recombinant fusion protein on thrombocytopoiesis in mice with thrombopenia inflicted by irradiation. Methods BALB／C mice weighting around 20 g were irradiated with 5 Gy of 60Coγ-ray irradiation to generate thrombopenia. The irradiation injured mice were injected with rTMP-GH or rhGH subcutaneously at the dose of 200 μg·kg－1·d－1 for 7 days. From the 6th day, the platelets in blood samples from vena caudalis were counted routinely, and the pathological changes of bone marrow were determined by morphological observation. Results From the 10th day, the levels of blood platelet in rTMP-GH treated mice were much higher than those of rhGH treatment group and normal saline (NS) control group, especially at the nadir ( P <0.01). On the 22nd day, the platelet count has recovered up to 80% of normal level in rTMP-GH treatment group, while it has just recovered up to 30% in NS control group. Morphological observation showed that there was obvious reconstruction of bone marrow in mice treated with rTMP-GH, compared with NS group.The number of megarkaryoblasts and megakaryocytes in bone marrow of rTMP-GH treated mice (3.07±0.32) was much higher than those of rhGH treatment group (2.20±0.22, P <0.05) and NS control group (0.87±0.19, P <0.01). Conclusions rTMP-GH has potent effects on the recovery of blood platelet by promoting megarkaryocytopoiesis in irradiation injuried mice. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
