| WANG Lei,LI Jin,JIANG En-hai,SONG Li,LIU Qiang,ZHANG Ning,TANG Wei-sheng,WANG Qin.Effects of human Rb94 gene transfer combined with γ-ray radiation on growth of esophageal carcinoma cells in vitro[J].Chinese Journal of Radiological Medicine and Protection,2009,29(6):568-570,589 |
| Effects of human Rb94 gene transfer combined with γ-ray radiation on growth of esophageal carcinoma cells in vitro |
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| DOI：10.3760/cma.j.issn.0254-5098.2009.06.003 |
| KeyWords:Retinoblastoma 94 gene Esophageal carcinoma Radiation |
| FundProject:国家自然科学基金(30770638) |
| Author Name | Affiliation | E-mail | | WANG Lei | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | LI Jin | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | JIANG En-hai | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | SONG Li | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | LIU Qiang | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | ZHANG Ning | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | TANG Wei-sheng | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | | | WANG Qin | Institute of Radiation Medicine, Chinese Academy of Medical Sciences, Peking Union Medical College, Tianjin Key Laboratory of Molecular Nuclear Medicine, Tianjin 300192, China | wangqin-1005@yahoo.com.cn |
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| Abstract:: |
| Objective To study the combined effect of foreign retinoblastoma 94 gene and ionizing radiation on the growth of esophageal carcinoma cells K150. Method K150 cells was transfected by recombinant Rb94 gene adenovirus vector and irradiated by 6 Gy137Csγ-ray after transfection. The cohorts were divided into blank control,Ad-lacZ,Ad-Rb94,irradiation and Ad-Rb94 combined with irradiation. Cell growth, cell cycle, cell apoptosis and expression of retinoblastoma 94 gene of K150 cells were detected. Results The growth of K150 cells transfected with Ad-Rb94, radiation and Ad-Rb94 combined with irradiation group at 7 day after infection was all inhibited. The group of Ad-Rb94 combined with irradiation resulted in greater inhibition of cells growth, reached 45.49%, compared with Ad-Rb94 infection(20.26%)and radiation group(35.11%)(χ2=14.25,P<0.05). Cells of G1 phases and apoptosis of K150 cells for the Ad-Rb94 combined with irradiation group were the highest, reached 73.4% and 15.7%, respectively.The combination of Ad-Rb94 and irradiation resulted in the greatest expression of retinoblastoma 94 gene, which was 1.87 fold and 2.92 fold of Ad-Rb94 infection and irradiation, respectively. Conclusions Ad-Rb94 gene transfection combined with irradiation shows the synergism for the inhibition of esophageal cancer K150 cells growth. |
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