| WANG Hong,LIU Qiang,YANG Yong-hua,WANG Lei,JIANG En-hai,FAN Fei-yue.Time effectiveness of DNA injury and its repair induced by irradiation using single cell gel electrophoresis[J].Chinese Journal of Radiological Medicine and Protection,2009,29(6):564-567 |
| Time effectiveness of DNA injury and its repair induced by irradiation using single cell gel electrophoresis |
| Received:May 25, 2009 |
| DOI：10.3760/cma.j.issn.0254-5098.2009.06.002 |
| KeyWords:Single cell gel electrophoresis DNA damage and repair DNA double-strand break |
| FundProject:国家自然科学基金(30800281);天津市自然科学基金(07JCYBJC09200) |
| Author Name | Affiliation | E-mail | | WANG Hong | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | LIU Qiang | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | lq6688@yahoo.com.cn | | YANG Yong-hua | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | WANG Lei | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | JIANG En-hai | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | | | FAN Fei-yue | Tianjin Key Laboratory of Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin 300192, China | |
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| Abstract:: |
| Objective To improve the accuracy of estimation of radiation biodosimetry after radiation accident, to explore the time-effect relationship of DNA repair after irradiation, and to describe the curves and plane of DNA repair. Methods The neutral single cell gel electrophoresis (SCGE) was performed to estimate the repair of DNA double-strand break (DNA-DSB) in human lymphocytes induced by different dose of radiation at 3, 24, 48, 72 h post irradiation. Results Better goodness of fit was obtained not only in the dose-response curves at different time points, but also in the time-response curves at different doses. A smooth three-dimensional plane model was obtained when combining the two curves. Conclusions The curves of DNA-DSB repair showed a linear relationship, and repair of radiation-plane model could be used for biological dosimetry. |
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