| HAO Shu-xia,ZHANG Wei,WANG Chun-yan,QI Xue-song,LV Hui-min.Effect of Auduolin on apoptosis and expression of Bax and Bcl-2 in spermatogenic cells of rats exposed to high power microwave[J].Chinese Journal of Radiological Medicine and Protection,2009,29(5):548-551 |
| Effect of Auduolin on apoptosis and expression of Bax and Bcl-2 in spermatogenic cells of rats exposed to high power microwave |
| Received:November 26, 2008 |
| DOI： |
| KeyWords:ADL High power microwave Apoptosis Bcl-2/Bax |
| FundProject: |
| Author Name | Affiliation | E-mail | | HAO Shu-xia | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | ZHANG Wei | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | WANG Chun-yan | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | QI Xue-song | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | LV Hui-min | National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | lu-huimin@163.com |
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| Abstract:: |
| Objective To investigate the protection function of Anduolin (ADL) against exposure to high power microwave, and to probe the mechanism involved. Methods The SPF-class male rats were randomly divided into ADL groups (Dose of 3, 6 and 9 g/kg body weight), model group and control group. Rats in ADL groups were continuously ingested with ADL for 20 d. The rats in ADL groups and model group were exposed to high power microwave at average power density of 100 mW/cm2 for 10min. Their testes were excised at 24 h, 48 h and 5 d after exposure to microwave. Apoptosis of spermatogenic cells and the expression of Bax and Bcl-2 were detected with in situ end-labeling method and immuno-histochemistry method, respectively. Results At 24 h,48 h and 5 d after exposure to high power microwave, compared with control group, the counts of apoptotic spermatogenic cells in exposed model group were increased significantly(t=-41.89,-11.29 and -62.24,P<0.05), while the ratio of Bcl-2/Bax in exposed group was decreased significantly(t=8.49,4.36 and 4.47, P<0.05). At the same time, compared with the exposed group, the counts of apoptotic spermatogenic cells in 3, 6, and 9 g/kg ADL groups were reduced significantly(F=5.25,9.79 and 15.35, P<0.05), while the ratio of Bcl-2/Bax was enhanced significantly(F=20.17,11.75 and 11.98, P<0.05). Conclusions The high power microwave could induce the apoptosis of spermatogenic cells. ADL protect the spermatogenic cells via the ratio Bcl-2/Bax increasing. |
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