| ZHANG Li-yuan,WU Yong-you,ZHANG Ming-zhi,et al.Low dose radiation induced bystander effect and its mechanism[J].Chinese Journal of Radiological Medicine and Protection,2009,29(5):483-485 |
| Low dose radiation induced bystander effect and its mechanism |
| Received:September 10, 2008 |
| DOI： |
| KeyWords:Low dose radiation Bystander effect Hormesis Superoxide radical c-fos |
| FundProject:苏州市科教兴卫基金资助项目(SWQ19) |
|
| Hits: 3834 |
| Download times: 2142 |
| Abstract:: |
| Objective To investigate whether the supernatant (the conditioned fluid) of myeloid cells suspension after low dose radiation (6 cGy) in vitro could result in hormesis on the normal or radiation damage cells and its mechanism. Methods Mice myeloid cell suspension was irradiated by 0, 2 and 5Gy, respectively, and cultured in vitro. MTT method was used to measure the reproductive activity of cells. Cytochrome C reduction method was used to determine the concentration of O-2, the immunohistochemical method to test the protein expression of c-fos. Results Co-cultured with the conditioned fluid, the reproductive activity of the myeloid cells after high dose irradiation(P<0.01),while both the concentration of O-2 and the protein expression of c-fos were enhanced(P<0.05). Conclusions The conditioned fluid could enhance the proliferation of the myeloid cells after radiation injury. The mechanism of the low dose radiation-induced bystander effect might be correlated with the increase of both the concentration of O－2 and the protein expression of c-fos. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
