| SHEN Guang-jian,TANG Wen-yuan,XU Min-hui,et al.Correlation between AQP4 mRNA and PKC activity after gamma knife radiosurgery in rat brain[J].Chinese Journal of Radiological Medicine and Protection,2009,29(5):479-482 |
| Correlation between AQP4 mRNA and PKC activity after gamma knife radiosurgery in rat brain |
| Received:November 25, 2008 |
| DOI： |
| KeyWords:Rat Gamma knife radiosurgery Aquaporin-4 Protein kinase C Calcium ion |
| FundProject: |
| Author Name | Affiliation | E-mail | | SHEN Guang-jian | Neurosurgical Department, Daping Hospital, Third Military Medical College, Chongqing 400042, China | | | TANG Wen-yuan | 重庆医科大学第一附属医院神经外科 | | | XU Min-hui | Neurosurgical Department, Daping Hospital, Third Military Medical College, Chongqing 400042, China | | | GENG Ming-ying | Neurosurgical Department, Daping Hospital, Third Military Medical College, Chongqing 400042, China | | | SUN Shan-quan | 重庆医科大学第一附属医院基础部解剖教研室 | sunsq2151@sohu.com |
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| Abstract:: |
| Objective To explore the change of AQP4 mRNA expression and the correlation with PKC in rat brain irradiated by γ knife radiosurgery (GKS). Methods 30 Wistar rats were used in the study. The experimental radiosurgery model was established by radiating rat left rotral caudate nucleus with GKS(one target,100 Gy in isocenter dose and 4 mm in collimator),and was examined at 1,3,7,15,30 and 45 d post-irradiation. AQP4 mRNA expression, PKC activity and free intracellular calcium ion concentration ([Ca2+]i) of brain tissue were determined by RT-PCR, liquid scintillation counter and Fura-2/AM, respectively. Results AQP4 mRNA expression increased gradually from 0.99±0.05 in control group to 2.32±0.10 at 30 d post-irradiation, and decreased to 2.21±0.08 at 45 d post-irradiation. The PKC activity and the free [Ca2+]i decreased gradually from 0.5896±0.2101 and 455.82±20.13 in control group to 0.0404±0.0294 and 196.72±9.87 at 30 d post-irradiation, and increased to 0.1050±0.0607 and 219.26±10.43 at 45 d post-irradiation, respectively. The significant differences were found between experimental group and control group except at 1 d post-irradiation (P<0.01). The correlation between AQP4 mRNA and the PKC activity was negative(P=0.003,r=-0.918), while that between the free [Ca2+]i and the PKC activity was positive(P=0.001,r=0.959). Conclusions The increased expression of AQP4 mRNA might result from the inhibition of PKC activity due to the reduction of free [Ca2+]i after GKS. |
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