| SUN Yuan-lin,TANG Jian,GU Xiao-hong,et al.Effect of polysaccharides from Angelica sinensis on Bcl-2 and Bax protein expression of irradiated liver cells[J].Chinese Journal of Radiological Medicine and Protection,2009,29(5):476-478,482 |
| Effect of polysaccharides from Angelica sinensis on Bcl-2 and Bax protein expression of irradiated liver cells |
| Received:November 11, 2008 |
| DOI： |
| KeyWords:Angelica sinensis Polysaccharides Bcl-2 Bax Apoptosis Radioprotection |
| FundProject:山西省青年科研基金(2007021042) |
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| Abstract:: |
| Objective To investigate the effect of polysaccharides from Angelica sinensis (ASP3) on Bcl-2 and Bax protein expression of irradiated liver cells from mice. Methods Bcl-2 and Bax protein expression of liver cells in vitro exposed to 2.0 Gy rays were examined by using immunohistochemistry method. Results The expression of apoptosis-accelerating protein Bax in the irradiation group was enhanced obviously(70.83%),while apoptosis inhibiting protein Bcl-2 tended to decline(55.60%),with the statistically significant difference(P<0.01) compared with that of the control. ASP3 pretreatment could regulate Bcl-2 and Bax protein expression of liver cells,inhibiting Bax protein expression(64.14/58.37%) and increasing Bcl-2 protein expression(59.21%/67.45%).The differences between the high dosage(100 mg/L of ASP3) and the irradiation group were statistically significant(P<0.05). Conclusions ASP3 pretreatment could prohibit the apoptosis of radiation-damaged liver cells due to abnormal expression of Bcl-2 and Bax, and reduce the cell apoptosis by increasing Bcl-2/Bax protein expression so as to enhance the radiation endurance of liver cells. |
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