| GENG Chuan-ying,XU Bo,LI Hong-yan,et al.Effect of radiation combined withChinese medicinal monomers on Me180 cells[J].Chinese Journal of Radiological Medicine and Protection,2009,29(5):472-475 |
| Effect of radiation combined withChinese medicinal monomers on Me180 cells |
| Received:September 04, 2008 |
| DOI： |
| KeyWords:Radiosensitizing effect Telomerase Chinese medicinal monomers |
| FundProject: |
| Author Name | Affiliation | E-mail | | GENG Chuan-ying | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | XU Bo | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | LI Hong-yan | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | CHEN Zhi-hua | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | XIA Qi-sheng | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | XU Mei | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | LIU Xuan | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | | | LIU Yu-feng | 北京市大兴区人民医院口腔科 | | | XIANG Qing | Department of Biochemistry and Molecular Biology, China-Japan Friendship Institute of Clinical Medical Sciences, Beijing 100029, China | xiang-qing2003@yahoo.com.cn |
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| Abstract:: |
| Objective To observe the effect of radiation treatment combined withChinese medicinal monomers on the proliferation function, telomerase activity, expressions of apoptosis- and proliferation-related genes of Me180 cells. Methods Me180 cells were cultured in the medium with oleanolic acid, curcumin and allicin. The survival rates of cells were detected by the methods of MTT, the telomerase activity by the method of telomeric repeat amplification protocol (TRAP) and the apoptosis -and proliferation-related genes by the method of reverse transcriptase-PCR. Me180 cells were cultured in the medium with Chinese medicinal monomers,and exposed to X-ray irradiation and the survival rates were detected. Results The results of MTT showed that survival rates of tumor cells exposed to X rays in combination with oleanolic acid, curcumin and allicin were decreased significantly(t=2.81, 4.16, and 3.42,P<0.05). Chinese medicinal monomers inhibited the telomerase activity of Me180 cells and the inhibiting function changed with time. At 16 h, the telomerase activities of Me180 cells administered with oleanolic acid and allicin were reduced markedly (t=5.11 and 5.29,P<0.05). After 48 h, the telomerase activities returned to the normal level. The gene expressions of p21 and p16 in Me180 cells treated with oleanolic acid were 2.43 and 2.78 times higher than the control, respectively, while those of cyclin D1 and CDK4 were 56% and 41% of the control, respectively. Conclusions Chinese medicinal monomers could effectively kill tumor cells, inhibit the telomerase activity and the expression of proliferation-related genes, and enhance the radiosensitivity of tumor cells. |
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